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Demonstration of Bovine Viral Diarrhoea Virus Antigens in Cell Cultures and in Paraffin-embedded Tissue Sections by the Peroxidase-antiperoxidase (PAP) Technique Using Monoclonal Antibodies

Acta Veterinaria Scandinavica volume 30, pages 231–233 (1989)Cite this article

Abstract

The diagnosis of both bovine viral diarrhoea (BVD) and mucosal disease (MD) is usually made on the basis of characteristic clinical and pathological findings. The definitive etiological diagnosis by virus isolation is time consuming, expensive and elusive. Isolation of the virus in cell cultures is rather difficult since it has no characteristic cytopathic effect (CPE). Furthermore, many strains have no CPE at all. Due to these uncertainties, virus isolation trials are generally supported by additional tests (Radostits & Littlejohns 1988).

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Acknowledgments

The authors are grateful to Professor C. Rehbinder for his helpful advices. We acknowledge Ms. Gunnel Svedlund for providing the cell culture controls. We thank Ms. Cajsa Magnusson and Ms. Eva Westergren for the excellent technical assistance. This work was supported by the National Veterinary Institute, Uppsala, Sweden. Dr. Gi- meno is a fellowship holder from the National Scientific Research Council (CONICET), Argentina.

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  1. Department of Pathology and Virology, National Veterinary Institute, P. O. Box 7073, S 750 07, Uppsala, Sweden

    Katinka Belák, E. J. Gimeno & S. Belák

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  1. Katinka Belák
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  2. E. J. Gimeno
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  3. S. Belák
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Belák, K., Gimeno, E.J. & Belák, S. Demonstration of Bovine Viral Diarrhoea Virus Antigens in Cell Cultures and in Paraffin-embedded Tissue Sections by the Peroxidase-antiperoxidase (PAP) Technique Using Monoclonal Antibodies. Acta Vet Scand 30, 231–233 (1989). https://doi.org/10.1186/BF03548060

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Acta Veterinaria Scandinavica

ISSN: 1751-0147