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Induction of Nematode-Trapping Organs in the Predacious Fungus Arthrobotrys oligospora (Hyphomycetales) by Infective Larvae of Ostertagia ostertagi (Trichostrongylidae)

Acta Veterinaria Scandinavica volume 30, pages 77–87 (1989)Cite this article

Abstract

Laboratory experiments were designed to study the influence of temperature, concentrations of nematodes, oxygen tension, light, and nutrient levels, on the induction of nematode-trapping hyphal nets in the predacious fungus Arthrobotrys oligospora. When induced by infective Ostertagia ostertagi larvae, a maximum number of nets was produced at 20°C, at which temperature nets in surplus were produced at larval concentrations up to 1,000 larvae per cm2. A. oligospora did not produce nets in an anaerobic atmosphere containing 21 % CO2 (v/v), and net induction was suppressed to a certain degree by exposure to light. The composition of the medium had an important influence on the saprophytic growth and the net-forming capability of A. oligospora as a maximum number of nets was induced at a relatively low concentration of corn meal supporting the relatively sparse mycelium. It was shown that a proportion of trapping nets in A. oligospora maintained their trapping potential for more than 7 weeks when the temperature was below 25°C. Induction of nematodetrapping organs in A. oligospora is discussed in relation to control of infective nematode parasite larvae in cow pats.

References

  • Balan J, Krizkova L, Nemec P, Vollek V: Production of nematode-attracting and nematici- dal substances by predacious fungi. Folia Microbiol. 1974, 79, 512–519.

    Article  Google Scholar 

  • Barron GL: Predators and parasites of microscopic animals. In: Biology of Conidial Fungi (Cole GT, Kendrick K, eds.). Academic Press, New York 1981, 2, 167–200.

    Google Scholar 

  • Cooke RC: Ecological characteristics of nematode-trapping Hyphomycetes. I. Preliminary studies. Ann. appl. Biol. 1963, 52, 431–437.

    Article  Google Scholar 

  • Cooke RC: Ecological characteristics of nematode-trapping Hyphomycetes. II. Germination of conidia in soil. Ann. appl. Biol. 1964, 54, 375–379.

    Article  Google Scholar 

  • Eren J, Pramer D: Growth and activity of the nematode-trapping fungus Arthrobotrys cono- ides in soil. In: Microbial Ecology (Loutit MW, Miles JAR, eds.). Springer-Verlag, Berlin 1978, 121–127.

    Chapter  Google Scholar 

  • Gronvold J, Korsholm H, Wolstrup J, Nansen P, Henriksen SVAA: Laboratory experiments to evaluate the ability of Arthrobotrys oligospora to destroy infective larvae of Cooperia species, and to investigate the effect of physical factors on the growth of the fungus. J. Helminthol. 1985, 59, 119–125.

    Article  CAS  Google Scholar 

  • Gronvold J, Nansen P, Henriksen SVAA, Thylin J, Wolstrup J: The capability of the predacious fungus Arthrobotrys oligospora (Hyphomyce- tales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. J. Helminthol. 1988, 62, 271–280.

    Article  CAS  Google Scholar 

  • Hayes WA, Blackburn F: Studies on the nutrition of Arthrobotrys oligospora Fres. and A. robusta Dudd. II. The predaceous phase. Ann. appl. Biol. 1966, 58, 51–60.

    CAS  Google Scholar 

  • Jansson H-B, Nordbring-Hertz B: Interactions between nematophageous fungi and plant-parasitic nematodes: Attraction, induction of trap formation and capture. Nematologica. 1980, 26, 383–389.

    Article  Google Scholar 

  • Jorgensen RJ, Madsen K: Undersogelse af fæces- prover for lungeormlarver. (Examination of faecal samples for lungworm larvae). Dansk VetTidsskr. 1982, 65, 517–518.

    Google Scholar 

  • Lysek G, Nordbring-Hertz B: An endogenous rhythm of trap formation in the nematopha- gous fungus Arthrobotrys oligospora. Planta. 1981, 752, 50-53.

  • Mankau R: Soil fungistasis and nematophagus fungi. Phytopathology 1962, 52, 611–615.

    Google Scholar 

  • Mattiasson B, Johansson P-A, Nordbring-Hertz B: Host-microorganism interaction; Studies on the molecular mechanisms behind the capture of nematodes by nematophagous fungi. Acta Chem. Scand. 1980, 34, 539–540.

    Article  Google Scholar 

  • Nansen P, Gronvold J, Henriksen SVAA Wolstrup J: Predacious activity of the nematode-destroying fungus, Arthrobotrys oligospora, on preparasitic larvae of Cooperia oncophora and on soil nematodes. Proc. Helminthol. Soc. Wash. 1986, 53, 237–243.

    Google Scholar 

  • Nansen P, Gronvold J, Henriksen SVAA Wolstrup J: Interactions between the predacious fungus Arthrobotrys oligospora and third-stage larvae of a series of animal-parasitic nematodes. Vet. parasitol. 1988, 26, 329–337.

    Article  CAS  Google Scholar 

  • Nordbring-Hertz B: Peptide-induced morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. Physiol. Plant. 1973, 29, 223–233.

    CAS  Google Scholar 

  • Nordbring-Hertz B: Nematode-induced morphogenesis in the predacious fungus Arthrobotrys oligospora. Nematologica 1977, 23, 443–451.

    Article  Google Scholar 

  • Nordbring-Hertz B, Brink C: Qualitative characterization of some peptides inducing morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. Physiol. Plant. 1974, 31, 59–63.

    CAS  Google Scholar 

  • Nordbring-Hertz B, Mattiasson B: Action of a nematode-trapping fungus shows lectin-media- ted host-microorganism interaction. Nature 1979, 257, 477–479.

    Article  Google Scholar 

  • Nordbring-Hertz B, Odham G: Determination of volatile nematode exudates and their effects on a nematode-trapping fungus. Microb. Ecol. 1980, 6, 241–251.

    Article  CAS  Google Scholar 

  • Nordbring-Hertz B, Stâlhammer-Carlemalm M: Capture of nematodes by Arthrobotrys oligospora, an electron microscope study. Canad. J. Bot. 1978, 56, 1297–1307.

    Article  Google Scholar 

  • Olthof HA, Estey RH: A nematotoxin produced by the nematophagus fungus Arthrobotrys oligospora Fresenius. Nature 1963, 796, 514–515.

    Article  Google Scholar 

  • Olthof HA, Estey RH: Relation of environmental factors to growth of several nematophagous Hyphomycetes. Canad. J. Microb. 1965, 77, 939–946.

    Google Scholar 

  • Pramer D, Stoll NR: Nemin: a morphogenic substance causing trap formation by predaceous fungi. Science 1959, 729, 966–967.

    Article  Google Scholar 

  • Rose JH: Some observations on the free-living stages of Ostertagia ostertagi, a stomach worm of cattle. Parasitology 1961, 57, 295–307.

    Article  Google Scholar 

  • Shepherd AM: Formation of the infection bulb in Arthrobotrys oligospora Fresenius. Nature 1955, 775, 475.

    Article  Google Scholar 

Download references

Acknowledgements

The author wishes to express his gratitude to Dr. Birgit Nordbring-Hertz, Lund university, Sweden, and to Dr. Michèle Peloille, INRA, Monnaie, France, for providing the fungal cultures.

Ms. Annette Kørner is thanked for valuable technical assistance. The author is also indebted to Ms. Jane Røken for correcting and typing the manuscript.

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  1. Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, 13, Bülowsvej, DK-1870, Frederiksberg C, Denmark

    Jørn Grønvold

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Grønvold, J. Induction of Nematode-Trapping Organs in the Predacious Fungus Arthrobotrys oligospora (Hyphomycetales) by Infective Larvae of Ostertagia ostertagi (Trichostrongylidae). Acta Vet Scand 30, 77–87 (1989). https://doi.org/10.1186/BF03548071

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Acta Veterinaria Scandinavica

ISSN: 1751-0147