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The First Salmonella Enteritidis Phage Type 1 Infection of a Commercial Layer Flock in Finland

Den första Salmonella Enterιtιdιs fagityp 1 ιnfektιonen i ett kommersiellt äggproducerande hönshus ι Fιnland

Acta Veterinaria Scandinavica volume 37, pages 471–480 (1996)Cite this article

Abstract

The first Salmonella Enteritidis phage type (PT) 1 infection in a commercial layer flock of 2700 birds in Finland occurred in 1995. All the birds were ordered to be killed, the eggs to be destroyed and access to the layer house was denied in order to prevent spread of the infection. Ninety one commercial layers, 61 replacement pullets and 1062 eggs were collected for the analyses. The total infection level of the flock was 8%, concentrated on the 2 older age groups. S. Enteritidis PT1 was isolated from livers (5%), ovaries (2%) and from caeca (3%), of which 2 positive samples were detected with pre-enrichment and 3 without pre-enrichment by cultivation Rambach agar. Eight % of 105 pooled egg samples were positive, of which 2 were detected only from contents and 3 only from shells indicating both oviductal and faecal contamination routes of eggs. The results support the use of the extended sampling procedure in poultry flocks suspected of human food-borne Salmonella outbreaks of invasive serotypes, including not only faecal but also environmental, organ, blood and/or egg samples.

Sammanfattning

Det första finländska Salmonella Enterιtιdιs fagιtyp (PT1) ιnfektιonsutbrottet ι en kommersiell äggproducerande hönsflock med 2700 fåglar har förekommιt och studerades. Alla fåglar slaktades, äggen förstördes och tillträde tιll farmen förbjöds för att undvιka spridandet av ιnfektιonen. 91 äggproducerande hönor, 61 unghönor och 1062 ägg samlades för analyserιng. Det totala ιnfektιonsstadιet ι flocken var 8% koncentrerat ι de 2 äldsta åldersgrupperna. S Enterιtιdιs PT1 ιsoledares från lever (5%), äggstockar (2%) och caecum (3%). Av de posιtιva caecum proven påvisades 2 med preannkning och 3 utan preannknιng på Rambach-agar. 8% av 105 samlade äggprov var posιtiva av vιlka 2 upptäcktes endast ι ιnnehållet och 3 ι skalen vιlket ιndιcerar både ovιductal och fe-kal kontammenng av äggen. Resultaten stöder användandet av en utvιdgad provtagnιngsprocedur ι fjä-derfaflockar som mιsstänks för humana lιvsmedel-burna Salmonella utbrott, bestående ιnte enbart av faecesprov utan även prov från mιljö, organ, blod och/eller ägg.

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Acknowledgments

The authors thank DVM Matti Salmela for his participation in the practical work. The technical assistance of the laboratory personnel of the Departments of Pathology and Field Extension, Bacteriology, Food Microbiology and Turku Regional Laboratory is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Food Microbiology, National Public Health Institute, FIN-00231, Helsinki, Finland

    T. M.-L. Johansson

  2. Department of Bacteriology, National Public Health Institute, FIN-00231, Helsinki, Finland

    R. Schildt

  3. Turku Regional Laboratory, National Public Health Institute, FIN-00231, Helsinki, Finland

    S. Ali-Yrkkö

  4. Laboratory of Enteric Pathogens, National Public Health Institute, FIN-00231, Helsinki, Finland

    A. Siitonen

  5. Department of Pathology and Field Extension, National Veterinary and Food Research Institute, Helsinki, Finland

    R. L. Maijala

Authors
  1. T. M.-L. Johansson
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  2. R. Schildt
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  3. S. Ali-Yrkkö
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  4. A. Siitonen
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  5. R. L. Maijala
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Johansson, T.ML., Schildt, R., Ali-Yrkkö, S. et al. The First Salmonella Enteritidis Phage Type 1 Infection of a Commercial Layer Flock in Finland. Acta Vet Scand 37, 471–480 (1996). https://doi.org/10.1186/BF03548087

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Acta Veterinaria Scandinavica

ISSN: 1751-0147