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Serological Characterization of Actinobacillus pleuropneumoniae Biotype 1 Strains Antigenically Related to both Serotypes 2 and 7
Serologisk karakterisering af Actinobacillus pleuropneumoniae biotype 1 stammer antigent beslægtet med serotype 2 og 7
Acta Veterinaria Scandinavica volume 37, pages 327–336 (1996)
Abstract
Nine Danish Actinobacillus pleuropneumoniae biotype 1 isolates were shown by latex agglutination and indirect haemagglutination to possess capsular polysaccharide epitopes identical to those of serotype 2 strain 1536 (reference strain of serotype 2) and strain 4226 (Danish serotype 2 strain). Immunodiffusion confirmed the antigenic relationship with serotype 2 and further demonstrated an antigenic relationship with strain WF83 (reference strain of serotype 7). SDS-PAGE with LPS from strains 1536, 4226, WF83 and strain 7317 (representative of the 9 isolates examined) showed that strains WF83 and 7317 had an identical smooth ladder pattern whereas LPS from strains 1536 and 4226 showed a distinctly different pattern. The antigenic similarities of the LPS of strains WF83 and 7317 were confirmed by immunoblots using rabbit or pig antisera prepared against the 3 strains. No antigenic similarities in the LPS of strains 1536 and 7317 were revealed. Since an antigenic determinant specific for the 9 isolates could not be demonstrated with the methods used, the strains are proposed to be designated K2:O7.
Sammendrag
Ni danske Actinobacillus pleuropneumoniae biotype 1 isolater viste sig ved latexagglutination og indirekte hæmagglutination at have kapsel polysaccharid epitoper fælles med serotype 2. Ved immunodiffusion bekræftedes det antigene slægtskab med serotype 2. Endvidere blev med denne test demonstreret et antigent slægtskab med serotype 7. SDS-PAGE af LPS fra stammerne 1536 (referencestamme for serotype 2), 4225 (dansk serotype 2), WF83 (referencestamme for serotype 7) og 7317 (repræsentant for de 9 stammer) viste et identisk mønster for WF83 og 7317, hvorimod LPS fra 1536 og 4226 viste et mønster, der var tydeligt forskelligt fra WF83 og 7317. At LPS fra stamme WF83 og 7317 var antigent identisk, blev yderligere bekræftet ved immunoblot med kanin eller grise antisera fremstillet mod de 2 stammer. Der var ikke antigent slægtskab mellem LPS fra stammerne 7317 og 1536. Da det ikke var muligt at påvise antigene determinanter, som var specifikke for de 9 isolater, foreslås det, at stammerne betegnes K2:O7.
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Nielsen, R., Andresen, L.O. & Plambeck, T. Serological Characterization of Actinobacillus pleuropneumoniae Biotype 1 Strains Antigenically Related to both Serotypes 2 and 7. Acta Vet Scand 37, 327–336 (1996). https://doi.org/10.1186/BF03548098
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DOI: https://doi.org/10.1186/BF03548098