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Effect of Timing of Frozen Semen Insemination on Pregnancy Rate in Mares

Inseminationstidpunktens inverkan på dräktighetsprocenten hos ston som inseminerats med fryst sperma

Acta Veterinaria Scandinavica volume 37, pages 361–365 (1996)Cite this article

Abstract

Thirty-four mares were inseminated with frozen semen from one stallion during 2 oestrous cycles, every 48 h until ovulation took place and within 12 h after ovulation. Semen was frozen using the Colorado method. The insemination dose was from 200 to 400×106 progressively motile spermatozoa. Ovaries were examined every 12 h to determine time of ovulation. Examination for pregnancy was carried out using ultrasonography, 15 days after ovulation. Thirty-five per cent of mares inseminated < 24 h and 23% of mares inseminated between 24 - 48 h before ovulation were pregnant (p = 0.388). The pregnancy rate in all mares inseminated before ovulation was 30%. In the mares inseminated within 12 h of ovulation, it was 18% (p = 0.253). Younger mares (aged 4-10 yr) had a higher pregnancy rate (59%) than older mares (aged 11-15 yr) (23%), but the difference was not statistically significant (p = 0.057).

Sammendrag

Trettiofyra ston inseminerades med fryst sperma från samma hingst under 2 brunstcykler; varannan dag före ovulationen och inom 12 timmar efter ovulationen. Sperman frystes enligt Colorado-metoden. Inseminationsdosen var 200–400 × 106 progressivt motila spermier. Äggstockarna undersöktes var 12:e timme för att fastställa ovulationstidpunkten. Dräktighetsundersökning utfördes med ultraljud 15 dagar efter ovulationen. Trettiofem procent av de ston som inseminerats < 24 h och 23% av de ston som inseminerats 24-48 h före ovulationen var dräktiga (p = 0.388). Dräktighetsprocenten hos samtliga ston som inseminerats före ovulationen var 30% och hos ston som inseminerats inom 12 timmar efter ovulationen 18% (p = 0.253). Yngre ston (4–10 år) hade en högre dräktighetsprocent (59%) än äldre ston (11–15 år) (23%), skillnaden var dock inte statistiskt signifikant (p = 0.057).

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Author notes

  1. M. Celebi

    Present address: Turkish Atomic Energy Authority, Lala- han Nuclear Research Institute in Animal Health, Ankara, Turkey

Authors and Affiliations

  1. Animal Reproduction, Faculty of Veterinary Medicine, University of Helsinki, Pohjoinen Pikatie 800, SF-04920, Saarentaus, Finland

    T. Katila & M. Celebi

  2. Equine Reproduction, Agricultural Research Centre, Jokioinen, Finland

    E. Koskinen

Authors
  1. T. Katila
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  2. M. Celebi
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  3. E. Koskinen
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Correspondence to T. Katila.

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Katila, T., Celebi, M. & Koskinen, E. Effect of Timing of Frozen Semen Insemination on Pregnancy Rate in Mares. Acta Vet Scand 37, 361–365 (1996). https://doi.org/10.1186/BF03548102

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Acta Veterinaria Scandinavica

ISSN: 1751-0147