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A Sperm Midpiece Defect in a Hereford Bull with Variable Semen Quality and Freezability
Mellomstykke-defekt hos spermier fra en Herefordokse med varierende sædkvalitet og spermiefrysbarhet
Acta Veterinaria Scandinavica volume 37, pages 367–373 (1996)
Abstract
A midpiece sperm defect with a frequency of 25-35% in ejaculates obtained from a Hereford bull with a 60 d non-retum rate of 76.4% after careful pre- and postfreeze semen selection was studied in light microscope and by transmission electron microscopy. The defect consisted in a folding and coiling of the distal midpiece characterized by disorganization and irregularity of mitochondria surrounding the axial fiber bundle, combined with retraction of doublet fibers and dislocation and fracturing of these elements and the corresponding dense fibers. Based on examination of the sper- matogenic epithelium it was concluded that the alterations in the axial fiber bundle were secondary to those in the mitochondrial sheath. The abnormality appeared to be related to the “Dag-like” defect earlier observed in different breeds.
Sammendrag
En spermie-defekt lokalisert til flagellens mellomstykke ble påvist med en frekvens på 25-35% i ejakulatene fra en Hereford-okse som ble brukt i produksjon av frossen sæd og hadde en 60 d NR% på 76,4 etter sterk seleksjon av sæden både før og etter frysing. Defekten, som ble undersøkt både i lys- og elektronmikroskop, besto i en uregelmessig organisering av mellomstykkets mitokondrier og i en refraksjon av de dobbelte mikrotubuli med påfølgende dislokasjon og fraktur både av disse og av de ytre fibre og bøyning av spermiehalen. Ultrastrukturelle undersøkelser av det spermatogenetiske epitel tydet på at forandringene i flagellens mikrotubuli og ytre fibre var sekundære til dem som ble påvist i mitokondriekappen, og at bøyningen av halen oppsto ved induksjon av motilitet under transport gjennom bitestikkelen. Klassifiseringen av defekten i forhold til de tidligere beskrevne “Dag” og “Dag-liknende” defekter hos ulike storferaser og defektens betydning for fruktbarheten blir diskutert.
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Andersen Berg, K., Filseth, O. & Engeland, E. A Sperm Midpiece Defect in a Hereford Bull with Variable Semen Quality and Freezability. Acta Vet Scand 37, 367–373 (1996). https://doi.org/10.1186/BF03548103
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DOI: https://doi.org/10.1186/BF03548103