Skip to main content
Download PDF
  • Published:

Atypical Aeromonas salmonicida Isolated from Diseased Turbot (Scophtalmus maximus L.)

Acta Veterinaria Scandinavica volume 37, pages 139–146 (1996)Cite this article

Abstract

Atypical Aeromonas salmonicida were isolated from 3 outbreaks of disease among farmed turbot (Scophthalmus maximus L.) in 3 different farms, 1 from Norway (Nl) and 2 from Denmark (DK1 and DK2). In all 3 cases, the incidence of disease and mortality was high and the main characteristic pathological finding was skin ulcers and septicaemia. The isolated bacteria were subjected to a thorough phenotypic and genotypic examination and comparison in the laboratory. All 3 isolates belonged to A. salmonicida but dis-played some very different biochemical properties. However, the 2 Danish strains, DK1 and DK2 had identical ribotypes but different from that of Nl, whereas the plasmid pro-files of DK1 and Nl were identical but different from that of DK2. These observations emphasize the need for an improvement of our understanding of the taxonomy and epi-demiology of atypical A. salmonicida.

References

  • Austin B, Austin DA: Bacterial Fish Pathogens: Dis-ease in Farmed and Wild Fish. 2nd ed. pp. 86–150. Ellis Horwood Ltd., Chichester, England, 1993

    Google Scholar 

  • Austin DA, Mcintosh D, Austin B: Taxonomy of fish associated Aeromonas spp., with the description of Aeromonas salmonicida subsp. smithia subsp. nov. Syst. Appi. Microbiol. 1989, 77, 277–290.

    Article  Google Scholar 

  • Bast L, Daly JG, DeGrandis SA, Stevenson BMW: Evaluation of profiles of Aeromonas salmonicida as epidemiological markers of furunculosis in-fections in fish. J. Fish Dis. 1988, 11, 133–145.

    Article  CAS  Google Scholar 

  • Chapman PF, Cipriano RC, Teska JD: Isolation and phenotypic characterization of an oxidase-nega-tive Aeromonas salmonicida causing furunculo-sis in coho salmon (Oncorhynchus kisutch). J. Wildlife Dis. 1991, 27, 61–67.

    Article  CAS  Google Scholar 

  • Dalsgaard I, Paulsen H: Atypical Aeromonas salmo-nicida isolated from diseased sand-eels, Ammod-ytes läncea (Cuvier) and Hyperoplus lanceolatus (Lesauvage). J. Fish Dis. 1986, 9, 361–364.

    Article  Google Scholar 

  • Enger O: Microbial ecology of marine fish farms, with special emphasis on the fish pathogenic bac-teria Vibrio salmonicida and Aeromonas salmo-nicida. Dr. Scient thesis. University of Bergen, Norway, 1991.

    Google Scholar 

  • Gravningen K, Dydland M: Sjukdom hos piggvar (Scophthalmus maximus L.). Norsk Veterinaer-tidsskrift 1991, 705, 923–926.

    Google Scholar 

  • Husevàg B, Lunestad BT, Johannesen PJ, Enger O, Samuelsen OB: Simultaneous occurrence of Vi-brio salmonicida and antibiotic resistant bacteria in sediments at abandoned aquaculture sites. J. Fish Dis. 1991,74 631–640.

    Article  Google Scholar 

  • Husevàg B, Lunestad BT: Presence of the fish patho-gen Aeromonas salmonicida and bacteria resist-ant to antimicrobial agents in sediments from Norwegian fish farms. Bull. Eur. Ass. Fish Pathol. 1995, 15, 17–19.

    Google Scholar 

  • Kado CI, Liu S-T: Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 1981, 745, 1365–1373.

    Article  Google Scholar 

  • Larsen JL, Mellergaard S: Microbiological and hy-gienic problems in marine aquaculture: Furuncu-losis and vibriosis in rainbow trout (Salmo gaird-neri). Bull. Eur. Ass. Fish Pathol. 1981, 7, 29–32.

    Google Scholar 

  • Laxdal B: A histopathological comparison of A. sal-monicida subsp. salmonicida infection in Atlan-tic salmon (Salmo salar L.) with its ECP and a proteolytic ECP fraction. M.Sc. thesis. The Insti-tute of Aquaculture, University of Stirling, Stirling, Scotland, 1989.

    Google Scholar 

  • LillehaugA, Lunder T, Poppe TT: Field testing of ad-juvanted furunculosis vaccines in Atlantic sal-mon (Salmo salar L.). J. Fish Dis. 1992, 15, 485–596.

    Article  Google Scholar 

  • Nielsen B, Olsen JE, Larsen JL: (1993) Plasmid pro-filing as an epidemiological marker within Aero-monas salmonicida. Dis. Aquat. Org. 1993, 15, 129–135.

    Article  Google Scholar 

  • Nielsen B, Olsen JE, Larsen JL: Restriction endonu-clease analysis (REA) and ribotyping of Aeromo-nas salmonicida. Dis. Aquat. Org. 1994, 18, 155–158.

    Article  Google Scholar 

  • Pedersen K, Kofod H, Dalsgaard I, Larsen JL: Isola-tion of an oxidase negative Aeromonas salmonic-ida from diseased turbot Scophthalmus maximus. Dis. Aquat. Org. 1994, 18, 149–154.

    Article  Google Scholar 

  • Pedersen K, Larsen JL: rRNA gene restriction pat-tern analysis of Vibrio anguillarum serogroup Ol. Dis. Aquat. Org. 1993, 16, 121–126.

    Article  CAS  Google Scholar 

  • Pedersen K, Dalsgaard I, Larsen JL: Characteriza-tion of atypical Aeromonas salmonicida isolates by ribotyping and plasmid profiling. J. appi. Bac-teriol. 1996, 80, 37–44.

    Article  CAS  Google Scholar 

  • Popoff M: Genus III. Aeromonas. Kluyver and Van Niel 1936, 398. In: Krieg NR, Holt JC (eds): Bergey’s Manual of Systematic Bacteriology, Vol. I, pp. 545–548. Baltimore: Williams & Wil-kins, 1984.

    Google Scholar 

  • VethaakAD: Diseases of flounder (Platichthys flesus L.) in the Dutch Wadden sea, and their relation to stress factors. Neth. J. Sea Res. 1992, 29, 257–272.

    Article  Google Scholar 

  • Wiklund T: Atypical Aeromonas salmonicida iso-lated from ulcers of pike, Esox lucius L. J. Fish Dis. 1990, 13, 541–544.

    Article  Google Scholar 

  • Wiklund T, Bylund G: A cytochrome oxidase negative bacterium (presumptively an atypical Aeromonas salmonicida) isolated from ulcerated flounders (Platichthys flesus (L.)) in the northern Baltic Sea. Bull. Eur. Ass. Fish Pathol. 1991, 11, 74–76.

    Google Scholar 

  • Wiklund T, Dalsgaard I: Atypical Aeromonas salmo-nicida associated with ulcerated flatfish species in the Baltic Sea and the North Sea. Dis. Aquat. Org. 1995 (in press).

    Google Scholar 

  • Wiklund T, Dalsgaard I, Eerola E, Olivier G: Charac-teristics of “atypical”, cytochromeoxidase-nega-tive Aeromonas salmonicida isolated from ulcer-ated flounders (Platichthys flesus (L.)). J. Appi. Bacteriol. 1994, 76, 511–520.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by The Danish Agricultural and Veterinary Research Council, grant no. 13-4710-1. The technical assistance of Mrs. M. HØjgård is gratefully appreciated.

Author information

Authors and Affiliations

  1. Laboratory of Fish Diseases, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Frederiksberg C, Denmark

    J. L. Larsen & K. Pedersen

Authors
  1. J. L. Larsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Pedersen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. L. Larsen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Larsen, J.L., Pedersen, K. Atypical Aeromonas salmonicida Isolated from Diseased Turbot (Scophtalmus maximus L.). Acta Vet Scand 37, 139–146 (1996). https://doi.org/10.1186/BF03548106

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548106

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147