Hyaluronan Turnover in the Synovial Fluid in Metacarpophalangeal–and Middle Carpal Joints in Standardbred Horses
Acta Veterinaria Scandinavica volume 37, pages 147–151 (1996)
The biological turnover of hyaluronan (sodium hyaluronate) of different molecular weights (0.6×106 and 2.5×106 Daltons) was studied in the synovial fluid of the middle carpal and metacarpophalangeal joints of 6 clinically healthy Standardbred horses. The hyaluronan was radioactively labelled with 14C. The biological half-life (t1/2) was calculated from repeated synovial samples after injection of the labelled hyaluronan. The mean t1/2 in the metacarpophalangeal joints was 9.7 h for low molecular weight hyaluronan and 8.9 h for high molecular weight hya-luronan and in the middle carpal joints 16.0 h and 12.5 h respectively. There was no sig-nificant difference in turnover of the different molecular weights of hyaluronan.
Aviad AD, Arthur RM, Brencick VA, Ferguson HC, Teigland MB: Synacid vs. Hylartin V in equine joint disease. J. Equine vet. Sci. 1988, 8, 112–116.
Balais EA, Watson D, Duff IE, Roseman S: Hyalu-ronic acid in synovial fluid. I. Molecular parame-ters of hyaluronic acid in normal and arthritic hu-man fluids. Arthritis Rheum. 1967, 10, 357–376.
Brown TJ, Laurent UBG, Fraser JRE: Turnover of hyaluronan in synovial joints: elimination of la-belled hyaluronan from the knee joint of the rab-bit. Exp. Physiol. 1991, 76, 125–134.
Dahl LB, Dahl IM, Engström-Laurent A, Granath K: Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies. Ann. Rheum. Dis. 1985, 44, 817–822.
Ekman L, Nilsson G, Persson L, Lumsden LH: Vol-ume of the synovia in certain joint cavities in the horse. Acta vet. scand. 1981, 22, 23–31.
Greenwald RA, Moy WW: Effects of oxygen-derived free radicals on hyaluronic acid. Arthritis Rheum. 1980, 23, 455–463.
Heinegãrd D, Inerot S, Wieslander J, Lindblad G: A method for the quantification of cartilage prote-oglycan structures liberated to the synovial fluid during developing degenerative joint disease. Scand. J. Clin. Lab. Invest. 1985, 45, 421–427.
Heldin P, Laurent TC, Heldin C-H: Effect of growth factors on hyaluronan synthesis in cultured hu-man fibroblasts. Biochem. J. 1989, 258, 919–922.
Laurent TC, Fraser JRE: Hyaluronan. The FASEB J., 1992, 6, 2397–2404.
Laurent TC, Fraser JRE: The properties of and turn-over of hyaluronan. In: Functions of proteogly-cans, Ciba Foundation Symp. Wiey, Chichester, England. 1986, 124, 9–29.
Laurent UBG, Fraser JRE, Laurent TC: An experi-mental technique to study the turnover of concen-trated hyaluronan in the anterior chamber of the rabbit. Exp. Eye Res. 1988, 46, 49–58.
Laurent UBG, Reed RK: Turnover of hyaluronan in the tissues. Adv. Drug Deliv. Rev. 1991, 7, 237–256.
Muir H: Current and future trends in articular cartil-age research and osteoarthritis. In: Knetter K E, Schleyerbach R, Hascall V C (eds.).: Articular cartilage Biochemistry. Raven Press, New York, 1986,423–440.
Phillips MW: Clinical trial comparison of intraartic-ular sodium hyaluronate products in the horse. J. Equine Vet. Sci. 1989, 9, 39–40.
Radin EL, Paul IL, Swann DA, Schottstaedt ES: Lu-brication of synovial membrane. Ann. Rheum. Dis. 1971, 30, 322.
Reed RK, Laurent UBG, Fraser JRE, Laurent TC: Removal rate of (3H)hyaluronan injected subcu-taneously in rabbits. Amer. J. Physiol. 1990, 259, 532–535.
Roneus B, Lindblad G, Lindholm A, Jones B: Effects of intraarticular corticosteroid and sodium hyalu-ronate injections on synovial fluid production and synovial fluid content of sodium hyaluronate and proteoglycans in normal equine joints. J.Vet. Med. A. 1993, 40, 10–16.
Rydell NW, Butler J, Balazs E A: Hyaluronic acid in synovial fluid. VI. Effect of intraarticular injec-tion of hyaluronic acid on the clinical symptoms of arthritis in track horses. Acta vet. scand. 1970, 11, 139–155.
Smith MM, Gosh P: The synthesis of hyaluronic acid by human synovial fibroblasts is influenced by the nature of the hyaluronate in the extracellular environment. Rheumatol. Int., 1987, 7, 113–122.
Swann DA, Radin EL, NazimiekM, Weisser PA, Cur-ran N, Lewinnek G: Role of hyaluronic acid in joint lubrication. Ann. Rheum. Dis. 1974, 33, 318.
Venn G, Nietfield JJ, Duits AJ, Brennan FM, Arner E, Covington M, Billingham MEJ, Hardingham TE: Elevated synovial fluid levels of Interleukin-6 and tumor necrosis factor associated with early experimental canine osteoarthritis. Arthritis Rheum. 1993, 3d, 819–826.
Ãsheim À, Lindblad G: Intraarticular treatment of ar-thritis in race-horses with sodium hyaluronate. Acta vet. scand. 1976, 17, 379–394.
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Lindholm, A., Ronéus, B., Lindblad, G. et al. Hyaluronan Turnover in the Synovial Fluid in Metacarpophalangeal–and Middle Carpal Joints in Standardbred Horses. Acta Vet Scand 37, 147–151 (1996). https://doi.org/10.1186/BF03548107