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Effects of Mild Mitral Valve Insufficiency, Sodium Intake, and Place of Blood Sampling on the Renin-Angiotensin System in Dogs

Acta Veterinaria Scandinavica volume 37, pages 109–118 (1996)Cite this article

Abstract

In 23 Cavalier King Charles Spaniels, 12 with mild mitral valve insufficiency (MVI) and 11 controls, the activity of the renin-angiotensin system (RAS) was assessed by measuring the plasma renin activity (PRA) and plasma aldosterone concentration (PAC) in 5 different settings. The dogs were sampled at the clinic before the trial and thereafter at home and at the clinic; during both a period on control diet and a period on low sodium diet. The dogs with mild MVI had the highest median PRA and PAC in all 5 settings. An analysis of variance accordingly showed that dogs with mild MVI had significantly higher PRA (p<0.0001) and PAC (p = 0.03) than controls, and that the sodium intake and place of blood sampling did not significantly affect this finding. The sodium intake had highly significant effects on PRA and PAC, and the place of blood sampling had no significant effects on PRA and PAC. The activity of angiotensin-converting enzyme in serum was lower in dogs with mild MVI than in controls (p = 0.0002). The plasma levels of endothelin-1, atrial natriuretic peptide, and arginine vasopressin, 3 peptides of pathophysiologic importance in congestive heart failure, were not significantly changed by the disease.

The early activation of the RAS in dogs with MVI suggests that the valvular disease process itself might be the cause of the activation, but confirmation of this requires further studies.

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Acknowledgments

The author wish to thank Professor Knud Poulsen, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University (RVAU) for providing laboratory facilities and reagents for the measurement of PRA. Associated professor Peter Bie, Department of Medical Physiology, University of Copenhagen is gratefully acknowledged for measuring ET-1, ANP and AVP. Assistant professor Annette Ersbøll, Department of Mathematics and Physics, RVAU is acknowledged for assisting with the statistical analyses. The excellent technical assistence of Ms Karin Faber and Mrs Susannne Holm Kristiansen, Department of Anatomy and Physiology, RVAU is highly appreciated. WALTHAM Centre for Pet Nutrition, Leicestershire, England is acknowledged for providing the diets. The study was supported by the Danish Agricultural and Veterinary Research Council project No. 13-4661 and 9400437, and by the Novo Nordic Fund.

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  1. Department of Anatomy and Physiology, Section for Veterinary Physiology and Biochemistry, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Frederiksberg C, Denmark

    H. D. Pedersen

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Pedersen, H.D. Effects of Mild Mitral Valve Insufficiency, Sodium Intake, and Place of Blood Sampling on the Renin-Angiotensin System in Dogs. Acta Vet Scand 37, 109–118 (1996). https://doi.org/10.1186/BF03548123

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Acta Veterinaria Scandinavica

ISSN: 1751-0147