Characterization and Identification of Caprine, Genital Mycoplasmas

Abstract

A total of 55 mycoplasma strains, isolated from the vagina of goats, were examined. Three strains, being arginine positive and glucose negative, could not be finally classified. Five isolates were identified as Acholeplasma laidlawii. Fourty-seven strains were phosphatase positive, glucose and arginine negative. Nine of these formed “film and spots” on standard growth medium, and reduced tetrazolium aerobically. Serological examination identified 7 as M. agalactiae, while 2 were M. bovis. The remaining 38 isolates did not reduce tetrazolium aerobically, and did not produce “film and spots.” on standard growth medium. All these except one were identified as M. bovigenitalium by immunofluorescence. Their relationship to group 11 otf Al-Aubaidi is discussed.

Sammendrag

Femoghalvtreds stammer dyrket fra vagina af geder undersøgtes med henblik på identifikation. Tre stammer, der var argininpositive og glukosenegative, kunne ikke endeligt klassificeres. Fem stammer fandtes at være Acholeplasma laidlawii. Syvogfyrre isolater var phosphatase positive, glukose- og argininnegative. Ni af disse dannede “film and spots” på standard vækstmedium og reducerede tetrazolium aerobt. Serologisk undersøgelse viste, at 7 af disse var Mycoplasma agalactiae, og 2 var M, bovis. De 38 resterende stammer dannede ikke “film and spots” på standardmediet og reducerede ikke tetrazolium aerobt. Alle på nær een af disse kunne på grundlag af biokemiske resultater og indirekte immunofluorescens artsbestemmes til M. bovigenitalium. Dette indebærer dog ikke, at de er helt identiske med bovine stammer af samme species. Relationen til ovin/caprin gruppe 11 (Al-Aubaidi) diskuteres.