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Attempts to Stimulate Mitosis in Cultured Trophoblast Cells of Cattle Embryos

Försök att stimulera mitos hos odlade trofoblastceller från nötkreatursembryon

Acta Veterinaria Scandinavica volume 27, pages 560–565 (1986)Cite this article

Abstract

Trophoblast from Day-14 bovine embryos was cultured in medium containing mitogens to determine if the mitotic index could be altered. Trophoblast from each of 15 embryos was cultured in minimum essential medium (Eagles) with 20 % fetal calf serum (control) or in this medium supplemented with pokeweed mitogen (1 %, v/v), phytohemagglutinin (1 %, v/v), concanavalin A (1 %, v/v) or thymidine (2 mg/ml). No mitogenic effect was observed due to any of the treatments. However, mitotic indexes were significantly lower when pokeweed (P < 0.05) or thymidine (P < 0.01) was added to the medium. A highly significant (P < 0.001) variation in mitotic index between embryos was observed.

Sammanfattning

KU lågt mitotiskt index hos odlade nörkreatursembryon begränsar bl a möjligheten till könsbestämning av embryon på cytogenetisk väg.

Syftet med försöket var att undersöka olika mitosstimulerande ämnens effekt pä odlade trofoblastceller från 14 dagar gamla nötkreatursembryon. Trofoblast från vardera 1–5 embryon delades i 5 delar som odlades i Minimum Essential Medium med tillsats av 20 % fetalt kalvserum (kontroll) och med tillsats av fyra olika mitogena substanser: Pokeweed, Phytohemagglutinin, Concanavalin A eller Thymidin. Ingen av substanserna visade sig ha någon mitosstimulerande effekt. Mitosindex var signifikant lägre vid. tillsats av Pokeweed (P < 0.05) och Thymidin (P < 0.01) jämfört med kontrollen. Variationen i mitosindex mellan embryon var höggradigt signifikant (P < 0.001).

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Acknowledgments

The technical aid of Ms. Ginette Pineault and Mireille Blacquiere and the financial support of the Knut and Alice Wellenberg fund and the Natural Sciences and Engineering Research Council of Canada are gratefully acknowledged.

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Authors and Affiliations

  1. Swedish Association for Livestock Breeding and Production, S-63184, Eskilstuna, Sweden

    H. Gustafsson, W.A. King, B. Berglund & L. Picard

  2. Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    H. Gustafsson, W.A. King, B. Berglund & L. Picard

  3. Centre de Recherche en Reproduction Animate, Fac. Med. Vet. Uni-versité de Montréal, Sl-Hyacinthe, Québec, Canada

    H. Gustafsson, W.A. King, B. Berglund & L. Picard

  4. Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden

    H. Gustafsson, W.A. King, B. Berglund & L. Picard

Authors
  1. H. Gustafsson
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  2. W.A. King
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  3. B. Berglund
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  4. L. Picard
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Gustafsson, H., King, W., Berglund, B. et al. Attempts to Stimulate Mitosis in Cultured Trophoblast Cells of Cattle Embryos. Acta Vet Scand 27, 560–565 (1986). https://doi.org/10.1186/BF03548135

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  • DOI: https://doi.org/10.1186/BF03548135

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Acta Veterinaria Scandinavica

ISSN: 1751-0147