Skip to main content
Download PDF
  • Published:

Ontogeny of T Cells in the Pig Fetus

Ontogenetisk udviklingsforløb for T celler i grisefostre

Acta Veterinaria Scandinavica volume 27, pages 343–350 (1986)Cite this article

Abstract

Mononuclear cells isolated from thymus, spleen and cord blood of pig fetuses ranging in age from 48 to 112 days were examined for the presence of sheep red blood cell rosette-forming cells (SRBC-RFC). After an initial increase from 77 % (mean) at 48 days of gestation to 88 % at 60 days, the proportion of SRBC-RFC in thymus remained constant throughout the gestational period. In spleen and cord blood, the proportion of SRBC-RFC increased with age, from occasional rosette-forming cells at 48 days of gestation to 21 % and 30 %, respectively, at 112 days. The demonstrated development of SRBC-RFC in the thymus, spleen and cord blood is considered to reflect the ontogeny of T cells in these fetal pig tissues.

Sammendrag

Mononukleære celler isoleret fra thymus, milt og navlestrengsblod fra grisefostre, varierende i alder fra 48 til 112 dage, blev undersøgt for tilstedeværelse af celler, som spontant danner rosetter med fåreerythrocytter (SRBG-RFG). I thymus steg andelen af SRBG-RFG innledningsvis fra 77 % (middelværdi) på 48. drægtighedsdag til 88% på 60. drægtighedsdag. Herefter forblev andelen af SRBG-RFG i thymus uforandret i den resterende del af drægtighedsperioden. I milt og navlestrengsblod steg andelen af SRBG-RFG i takt med fosteraider, fra enkelte rosettedaninende celler på 48. drægtighedsdag til henholdvis 21 % og 30 % på 112.-dagen. Udviklingen af SRBG-RFG i thymus, milt og navlestrengsblod, som er påvist i denne undersøgelse, anses for at afspejle det ontogenetiske udviklingsforløb for T celler i disse grisefosterorganer.

References

  • Binns, R.M.: Sheep erythrocyte rosettes in pigs, sheep, cattle and goats demonstrated in the presence of dextran. J. Immunol. Methods 1978, 21, 197–210.

    Article  CAS  Google Scholar 

  • Binns, R.M.: Organisation of the lymphoreticular system and lymphocyte markers in the pig. In: F. Kristensen and D. F. Antczak (Editors): Advances in Veterinary Immunology 1981. Elsevier, Amsterdam 1982, pp. 95–146.

    Google Scholar 

  • Buschmann, H. & S. Pawlas: A study of porcine lymphocyte populations. II. Characterization of porcine lymphocyte populations. Vet. Immunol. Immunopathol. 1980, 1, 225–241.

    Article  CAS  Google Scholar 

  • Bøyum, A.: Separation of blood leucocytes, granulocytes and lymphocytes. Tissue Antigens 1974, 4, 269–274.

    Article  Google Scholar 

  • Escajadillo, C. & R.M. Binns: Rosette formation with sheep erythrocytes — a possible T-cell marker in the pig. Int. Archs Allergy appl. Immun. 1975, 48, 201–275.

    Article  Google Scholar 

  • Ferguson, F.G., A. Pinto, J. Ritchie, L. Bailey & A. Zarkoiver: Porcine perinatal cell populations and cell-mediated immune responses. Proceedings of the International Pig Veterinary Society Congress, Copenhagen 1980, p. 185.

    Google Scholar 

  • Ford, W.L. & S.V. Hunt: The preparation and labelling of lymphocytes. In: D. M. Weir (Editor), Handbook of Experimental Immunology vol. 2. Cellular Immunology, 2nd ed. Blackwell, Oxford 1973, pp. 23.7–23.8.

    Google Scholar 

  • Hokland, P. & I. Heron: Analysis of the lymphocyte distribution during Isopaque-Ficoll isolation of mononuclear cells from human peripheral blood. J. Immunol. Methods 1980, 32, 31–39.

    Article  CAS  Google Scholar 

  • Jaroskova, L. & F. Kovaru: Identification of T and B lymphocytes in pigs by combined E-rosette test and surface Ig labelling. J. Immunol. Methods 1978, 22, 253–261.

    Article  CAS  Google Scholar 

  • Kaplan, M.E. & C. Clark: An improved rosetting assay for detection of human T lymphocytes. J. Immunol. Methods 1974, 5, 131–135.

    Article  CAS  Google Scholar 

  • Kaupp, E., R. Pabst & F. Trepel: Rosette formation of pig peripheral blood lymphocytes with sheep red blood cells. Z. Immun.- Forsch. 1977, 152, 438–446.

    CAS  Google Scholar 

  • Kovaru, F., & L. Jaroskova: Development of E-rosette formation in ontogeny of pigs. Folia Biologica (Prague) 1979, 25, 399–400.

    CAS  Google Scholar 

  • McCauley, L & P.E. Hartmann: Changes in the proportion and absolute number of T lymphocytes in piglets from birth until after weaning and in adults. Res. Vet. Sci. 1984, 37, 52–57.

    Article  CAS  Google Scholar 

  • Nielsen, J.: Mitogenic reactivity of mononuclear cells isolated from thymus, spleen and cord blood of pig fetuses. 1985, (submitted).

    Google Scholar 

  • Pabst, R., E. Kaupp & F. Trepel: Relative and absolute numbers of E- and EAC-rosette forming cells in lymphoid organs. Blut 1977, 34, 201–210.

    Article  CAS  Google Scholar 

  • Pellegrino, M.A., S. Ferrone, M.P. Dierich & R.A. Reisfeld: Enhancement of sheep red blood human lymphocyte rosette formation by the sulphydryl compound 2-amino ethylisothiouronium bromide. Clin. Immunol. Immunopathol. 1975, 3, 324–333.

    Article  CAS  Google Scholar 

  • Reyero, C., J.G. Thalhammer, G. Reszler & W. Stockl: Development of peripheral B and T lymphocytes in piglets. Z. Immun.- Forsch. 1978, 154, 409–415.

    CAS  Google Scholar 

  • Salmon, H.: Ontogeny of pig lymphocyte subpopulations. Proceedings of the International Pig Veterinary Society Congress, Copenhagen 1980, p. 179.

    Google Scholar 

Download references

Acknowledgements

I wish to thank Mrs. Hanne Petersen for skilful technical assistance. The project was partly supported by a grant from the European Economic Community under the programme “Protection of the young animal” (Contract No. 0161).

Author information

Authors and Affiliations

  1. State Veterinary Institute for Virus Research, Lindholm, DK-4771, Kalvehave, Denmark

    Jens Nielsen

Authors
  1. Jens Nielsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nielsen, J. Ontogeny of T Cells in the Pig Fetus. Acta Vet Scand 27, 343–350 (1986). https://doi.org/10.1186/BF03548148

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548148

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147