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Counter Current Line Absorption Immunoelectrophoresis is an Alternative Diagnostic Screening Test to Counter Current Immunoelectrophoresis in Aleutian Disease (AD) Eradication Programs

Modstrøms linie absorption immun-elektroforese er en alternativ diagnostisk screening-test til modstrøms-immun-elektroforese i minkplasmacytose udryddelsesprogrammer

Acta Veterinaria Scandinavica volume 27, pages 410–420 (1986)Cite this article

Abstract

Counter current immunoelectrophoresis (CCIE) is the diagnostic method used in the ongoing Aleutian disease virus eradication program on Danish mink farms. There has been an increasing demand for an alternative diagnostic test especially to evaluate suspected false positive CCIE reactions. We compared test results of a number of negative and positive mink sera in indirect counter current immunoelectrophoresis (ICCIE), counter current line absorption immunoelectrophoresis (CCLAIE) and radio immuno assay (RIA) with test results from counter current immunoelectrophoresis and found that counter current line absorption immunoelectrophoresis is the best alternative diagnostic screening test to counter current immunoelectrophoresis for Aleutian disease eradication programs. Not only proved the CCLAIE test to be useful for evaluation of doubtfully positive CCIE reactions, but it was found to have a higher sensitivity than the CCIE test.

Sammendrag

Modstrømselektroforese er den diagnostiske metode, der i dag brages i mink plasmacytose udryddelsesprogrammet for danske minkfarmen. Det har vist sig, at der er et stigende behov for en alternativ diagnostisk metode specielt til at benytte ved evaluering af mulige falske positive reaktioner i modstrømselektroforese. Vi sammenlignede analyseresultater fra 3321 negative og positive mink sera i den indirekte modstrømselektroforese, modstrøms linie absorption immunelektroforese og radio immun assay med analyseresultater fra modstrøms elektroforese og fandt, at modstrøms linie absorption immunelektroforese er det bedste alternativ til modstrømselektroforese i mink plasmacytose udryddelsesprogrammet. Modstrøms linie absorption immun-elektroforese viste sig ikke blot at være nyttig til evaluering af mulige mistænkelige positive reaktioner i modstrømselektroforese, men den viste sig også at have en større følsomhed end modstrømselektroforese.

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Acknowledgement

The technical help from mrs. Helle Klysner and miss Else Bang Larsen is greatly acknowledged. The study was supported from the Danish Fur Breeders Association Research Foundation.

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Authors and Affiliations

  1. Department of Veterinary Virology and Immunology and Department of Pathology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870, Frederiksberg C, Denmark

    Bent Aasted, Søren Alexandersen, Anders Cohn & Mogens Hansen

  2. Danish Fur Breeders Association, Glostrup, Denmark

    Bent Aasted, Søren Alexandersen, Anders Cohn & Mogens Hansen

Authors
  1. Bent Aasted
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  2. Søren Alexandersen
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  3. Anders Cohn
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  4. Mogens Hansen
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Aasted, B., Alexandersen, S., Cohn, A. et al. Counter Current Line Absorption Immunoelectrophoresis is an Alternative Diagnostic Screening Test to Counter Current Immunoelectrophoresis in Aleutian Disease (AD) Eradication Programs. Acta Vet Scand 27, 410–420 (1986). https://doi.org/10.1186/BF03548155

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Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147