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Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle
I. PCR Screening and Allele Frequency Estimation
Acta Veterinaria Scandinavica volume 34, pages 231–236 (1993)
Abstract
A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.
References
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Acknowledgments
We are grateful to Dr. M.E. Kehrli, Jr., The National Animal Disease Center, USDA-Agricultural Research Service, Ames, Iowa, USA for providing us with the protocol for the PCR assay and to Dr. D. Bailey, University of Cambridge, Cambridge, UK for the Hot-start hint. Thanks to Dr. T. Lykke, The Danish Agricultural Advisory Centre, Aarhus, Denmark for initiating the program. Thanks to Dr. B. Aasted, The Royal Veterinary and Agricultural University, Copenhagen, Denmark for the flow cytometric analysis and to Dr. K. Christensen, The Royal Veterinary and Agricultural University, Copenhagen, Denmark for the chromosome analysis. Last but not least thanks to Mrs. I. Christensen and Mrs. A.B. Nielsen for excellent technical assistance.
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Jørgensen, C., Agerholm, J., Pedersen, J. et al. Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle. Acta Vet Scand 34, 231–236 (1993). https://doi.org/10.1186/BF03548186
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DOI: https://doi.org/10.1186/BF03548186