Skip to main content
Download PDF
  • Published:

RNA Synthesis in Porcine Blastomere Nuclei Introduced into in Vitro Matured Ooplasm

Acta Veterinaria Scandinavica volume 34, pages 159–167 (1993)Cite this article

Abstract

The objective was to investigate the RNA synthesis in porcine blastomere nuclei upon transplantation into in vitro matured enucleated oocytes. Nuclei from 2- to 8-cell porcine embryos were introduced into the ooplasm of in vitro matured and enucleated porcine oocytes by electrofusion, and the resultant reconstructed embryos were cultured in vitro. Before fusion or at different intervals after this event embryos were incubated with [3H]-uridine, fixed, and histologically processed for autoradiography in order to detect RNA synthesis. About two thirds of the embryos were considered to depict normal development. All blastomeres displayed pronounced RNA synthesis before fusion, at 3 and 9 h after fusion the synthesis decreased or ceased, and at 24-49 h some embryos resumed synthesis at the 1- to 2-cell stage.

References

  • Bondioli KR, Westhusin ME, Looney CR: Production of identical bovine offspring by nuclear transfer. Theriogenology, 1990, 33: (165–174).

    Article  Google Scholar 

  • Camous S, Kopečný S, Eléchon JE: Autoradiographic detection of the earliest stage of (3H)uri-dine incorporation into the cow embryo. Biol. Cell. 1986, 58: (195–200).

    Article  CAS  Google Scholar 

  • Chesne P, Heyman Y, Peynot N, Desmedt V, Rao VH, Renard JP: Effect of in vitro or in vivo maturation of cattle oocytes on cleavage rate following nuclear transfer. 7th Scientific Meeting AETE 1991, p.136.

  • Collas P, Robl JM: Relationship between nuclear remodeling and development in nuclear transplant rabbit embryos. Biol. Reprod. 1991, 45: (455–465).

    Article  CAS  Google Scholar 

  • Grondahl C, Madison V, Hyttel P, Avery B, Nielsen J, Greve T: Onset of RNA synthesis in bovine embryos after maturation, fertilization, and development in vitro. ARTA 1990, 1: (135–142).

    Google Scholar 

  • Hyttel P, Madsen I: Rapid method to prepare mammalian oocytes and embryos for transmission electron microscopy. Acta anat. 1987, 129: (12–14).

    Article  CAS  Google Scholar 

  • Hyttel P, Prochazka R, Smith S, Kanka J, Greve T, Hoier R: Transcriptional activity of porcine blastomere nuclei introduced into in vitro matured ooplasm by electrically induced fusion. Theriogenology 1990, 33: 301.

    Article  Google Scholar 

  • Kanka J, Fulka J, Jr. Fulka J, Petr J: Nuclear transplantation in bovine embryo: Fine structural and autoradiographic studies. Mol. Reprod. Dev. 1991, 29:110–116.

    Article  CAS  Google Scholar 

  • Naish SH, Perrault SD, Zirkin BR: DNA synthesis following micro-injection of heterologous sperm and somatic cell nuclei into hamster oocytes. Gamete Res. 1987, 18: (109–120).

    Article  CAS  Google Scholar 

  • Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL: Nuclear transplantation in the bovine embryo: Assessment of donor nuclei and recipient oocyte. Biol. Reprod. 1987, 37: (859–866).

    Article  CAS  Google Scholar 

  • Prather RS, Sims ML, First NL: Nuclear transplantation in the early porcine embryo. Theriogenology 1988, 29: 290.

    Article  Google Scholar 

  • Prather RS, Sims MM, First NL: Nuclear transplantation in early pig embryos. Biol. Reprod. 1989, 41: (414–418).

    Article  CAS  Google Scholar 

  • Prather RS, Eichen PA, Nicks DK, Peters MS: Artificial activation of porcine oocytes matured in vitro. Mol. Reprod. Dev. 1991, 28: (405–409).

    Article  CAS  Google Scholar 

  • Procházka R, Smith S, Hyttel P, Greve T: Behavior of pig blastomere nuclei introduced into in vitro matured ooplasm by electrically induced fusion. Theriogenology 1990, 33: 301.

    Article  Google Scholar 

  • Robl JM, Prather R, Barnes F, Eyestone W: Nuclear transplantation in bovine embryos. J Anim. Sei. 1987, 64: (642–647).

    Article  CAS  Google Scholar 

  • Smith LC, Wilmut I: Factors affecting the viability of nuclear transplanted embryos. Theriogenology 1990, 33: (153–164).

    Article  Google Scholar 

  • Smith SD, Schmidt M, Purwantara B, Greve T: Oviduct epithelial cell-co-culture of early porcine embryos. Acta vet scand. 1992, 33: (349–355).

    CAS  PubMed  Google Scholar 

  • Stice SL, Robl JM: Nuclear reprogramming in nuclear transplant rabbit embryos. Biol. Reprod. 1988, 39: (657–664).

    Article  CAS  Google Scholar 

  • Szöllösi D, Czolowska R, Szöllösi MS, Tarkowski AK: Remodeling of mouse thymocyte nuclei depends on the time of their transfer into activated, homologous oocytes. J Cell. Sei. 1988, 91: (603–613).

    Google Scholar 

  • Tománek J, Kopečný V, Kanka J: Genome reactivation in developing early pig embryos: An ultra-structural and autoradiographic analysis. Anat. Embryol. 1989, 180: (309–316).

    Article  Google Scholar 

  • Willadsen SM: Nuclear transplantation in sheep embryos. Nature 1986, 320: (63–65).

    Article  CAS  Google Scholar 

  • Willadsen SM, Janzen RE, McAlister RJ, Shea BF, Hamilton G, McDermand D: The viability of late morulae and blastocysts produced by nuclear transplantation in cattle. Theriogenology 1991, 35: (161–170).

    Article  Google Scholar 

  • Yoshida M, Ishizaki Y, Kawagishi H: Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes matured in vitro. J. Reprod. Fert. 1990, 88: (1–8).

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Ms. Jytte Nielsen and Ms. Hanne Holm for preparing the autoradiographs and photographs, respectively. The work was supported by the Animal Biotechnology Research Centre.

Author information

Authors and Affiliations

  1. Department of Anatomy and Physiology and Department of Clinical Studies Reproduction, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Frederiksberg C, Denmark

    P. Hyttel, R. Procházka, S. Smith, J. Kaňka & T. Greve

  2. Department of Genetics, Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Libechov, Czech Republic

    P. Hyttel, R. Procházka, S. Smith, J. Kaňka & T. Greve

Authors
  1. P. Hyttel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Procházka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Kaňka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Greve
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hyttel, P., Procházka, R., Smith, S. et al. RNA Synthesis in Porcine Blastomere Nuclei Introduced into in Vitro Matured Ooplasm. Acta Vet Scand 34, 159–167 (1993). https://doi.org/10.1186/BF03548205

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548205

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147