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Identification of Actinobacillus Pleuropneumoniae Serotype 2 by Monoclonal or Polyclonal Antibodies in Latex Agglutination Tests

Acta Veterinaria Scandinavica volume 34, pages 223–225 (1993)Cite this article

Abstract

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a major problem in modern swine industry worldwide. At present 12 serotypes have been recognized (Nielsen 1990). Serotyping has been based upon capsule associated, heat-stabile antigens of polysaccharide (PS) and lipopolysaccharide (LPS) nature. A variety of tests have been used for serotyping including slide agglutination (Mittal et al. 1982), immunodiffusion (Gunnarsson et al. 1978, Nielsen & O’Connor 1984), indirect haemagglutination (Mittal et al. 1983a) and coagglutination (Mittal et al. 1983b). Recent studies report on serotyping of A. pleuropneumoniae strains using monoclonal antibodies in enzyme-linked immunosorbent assays (Lida et al. 1990, Nakai et al. 1990).

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Authors and Affiliations

  1. National Veterinary Laboratory, 27 Bülowsvej, DK-1790, Copenhagen V, Denmark

    Steen B. Giese, Eva Stenbœk & Ragnhild Nielsen

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  1. Steen B. Giese
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  2. Eva Stenbœk
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  3. Ragnhild Nielsen
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Giese, S.B., Stenbœk, E. & Nielsen, R. Identification of Actinobacillus Pleuropneumoniae Serotype 2 by Monoclonal or Polyclonal Antibodies in Latex Agglutination Tests. Acta Vet Scand 34, 223–225 (1993). https://doi.org/10.1186/BF03548215

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Acta Veterinaria Scandinavica

ISSN: 1751-0147