Skip to main content
Download PDF
  • Published:

Bovine Uterine, Cervical and Ovarian Cytosol Estrogen and Progesterone Receptor Concentrations in Cystic Ovarian Disease

Acta Veterinaria Scandinavica volume 34, pages 35–43 (1993)Cite this article

Abstract

Bovine cytosol estrogen (ERC) and progesterone receptor (PRC) concentrations were measured simultaneously in various regions of the uterus and in ovarian stromal tissue in cows with cystic ovarian disease (follicular cysts), arid the concentrations compared with those in animals with normal cycles. In cystic ovarian disease, ERC concentrations in endometrium (550 fmol/mg cytosol protein (c.p.)) and in myometrium (405) were significantly higher than in control animals. Very high PRC contents were measured in the endometrium (3115) and myometrium (2761) of cows with cystic ovarian disease. In control animals, PRC concentrations in the endometrium and myometrium were significantly lower than in diseased animals. No statistical differences were observed in ERC or PRC contents between the endometrium and the myometrium in cows with cystic ovarian disease. ERC and PRC concentrations in the uterine cervix and ovaries were low compared to those detected in the uterus. Bovine serum estradiol-17ß concentrations were higher (p<0.001) in cows with cystic ovarian disease than in control animals in postpartum anestrus or during the normal estrous cycle. Serum sex hormone-binding globulin (SHBG) concentrations were of the same magnitude as in control cows during their estrous cycles. These findings show that long standing low endogenous progesterone and elevated estradiol concentrations in serum are associated with elevated ERC and PRC concentrations in bovine uterus.

References

  • Apter D, Jänne O, Karvonen P, Vihko R: Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000. Clin. Chem. 1976, 22, 32–38.

    Article  CAS  Google Scholar 

  • Ax R, Bellen M, Schneider D, Haase-Hardie J: Reproductive Performance of Dairy Cows with Cystic Ovaries Following Administration of Procystin™1. J. Dairy Sci. 1986, 69, 542–545.

    Article  CAS  Google Scholar 

  • Bolton N, Ruokonen A, Vihko R: Stimulation of the synthesis of steroids and steroid sulphates in human testicular tissue in vitro by HCG and by 8-bromo-cyclic AMP. J. Biochem. 1985, 22, 481–485.

    CAS  Google Scholar 

  • Brown J, Schoenemann H, Reeves J: Effect of FSH treatment on LH and FSH receptors in chronic cystic-ovarian-diseased dairy cows. J. Anim. Sci. 1986, 62, 1063–1071.

    Article  CAS  Google Scholar 

  • Bäckström G, Edqvist L-E, Ekman L, Gustafsson B: Effect of LH- RH in cows with cystic ovaries: evaluation of a field study. Proc. 8th Int. Congr. on Anim. Reprod. A.I. 1976, 4, 548–551.

    Google Scholar 

  • Cantley T, Garverick H, Bierschwal C, Martin C, Youngquist R: Hormonal response of dairy cows with ovarian cysts to GnRH. J. Anim. Sci. 1975, 41, 1666–1673.

    Article  CAS  Google Scholar 

  • Clark J, Scrader W, O'Malley B: Mechanisms of steroid hormone action. In: Witson J, Fortec D (eds): Textbook of Endocrinology. W.B.Saunders Company, Philadelphia, 1985, 33–75.

    Google Scholar 

  • Dobson H, Rankin J, Ward W: Bovine cystic ovarian disease: Plasma hormone concentrations and treatment. Vet. Rec. 1977, 3, 459–461.

    Google Scholar 

  • Garverick H, Kesler D, Cantley T, Elmore R, Youngquist R, Bierschwal C: Hormone response of dairy cows with ovarian cysts after treatment with HCG or GnRH. Theriogenology 1976, 6, 413–425.

    Article  Google Scholar 

  • Gorodeski I, Geier A, Lunenfeld B, Beery B, Bahary C: Progesterone (P) receptor dynamics in estrogen primed normal human cervix following P injection. Fértil. Steril. 1987, 47,108–113.

    Article  CAS  Google Scholar 

  • Hammond G, Ahonen V, Vihko R: The radioimmunoassay of testosterone, 5a-dihydrotestosterone and their precursors in the human testis. J. Androl. (suppl.) 1978, 2, 391–399.

    Article  CAS  Google Scholar 

  • Hammond GL, Lähteenmäki PLA: A versatile method for the determination of serum Cortisol binding globulin and sex hormone binding globulin binding capacities. Clin. Chim. Acta 1983, 132, 101–110.

    Article  CAS  Google Scholar 

  • Jänne O, Apter D, Vihko R: Assay of testosterone, progesterone and 17a-hydroxyprogesterone in human plasma by radioimmunoassay after separation on hydroxyalkoxypropyl Sephadex. J. Steroid Biochem. 1974, 5, 155–162.

    Article  Google Scholar 

  • Katzenellenbogen B: Dynamics of steroid hormone action. Annu. Rev. Physiol. 1980,42,17–35.

    Article  CAS  Google Scholar 

  • Kesler D, Garverick H, Caudle A, Bierschwal C, Elmore R, Youngquist R: Clinical and endocrine responses of dairy cows with ovarian cysts to GnRH and PGF2α. J. Anim. Sci. 1978, 46, 719–725.

    Article  CAS  Google Scholar 

  • Kesler D, Garverick H, Caudle A, Elmore R, Youngquist R, Bierschwal C: Reproductive hormone and ovarian changes in cows with ovarian cysts. J. Dairy Sci. 1980, 63, 166–170.

    Article  CAS  Google Scholar 

  • Kesler DJ, Garverick HA: Ovarian cysts in dairy cattle: a review. J. Anim. Sci. 1982, 55, 1147–1159.

    Article  CAS  Google Scholar 

  • Kittok R, Britt J, Edgerton L: Serum steroid after gonadotropin treatment in cows with ovarian follicular cysts. Amer. J. vet. Res. 1974, 35, 1575–1576.

    CAS  PubMed  Google Scholar 

  • Kontula K: Progesterone-binding proteins from endometrium and myometrium of sheep uterus: a comparative study. Acta Endocr. (Kbh.) 1975, 78, 593–603.

    CAS  Google Scholar 

  • Koppinen J, Vesanen M, Alanko M: Ovarian cysts in dairy cattle - some aspects of diagnosis, treatment with GnRH and HCG and subsequent milk progesterone values. Nord. Vet.-Med. 1984, 36, 26–31

    CAS  PubMed  Google Scholar 

  • Leavitt W, MacDonald R, Okulicz W: Hormonal regulation of estrogen and progesterone receptor systems. In: Litwack G (ed): Biochemical Actions of Hormones. Academic Press, New York, 1983, 32–356.

    Google Scholar 

  • Leinonen P: Estrone and estradiol concentrations in the testis and spermatic and peripheral venous blood of elderly men: the influence of estrogen treatment. J. Steroid Biochem. 1980, 13, 737–742.

    Article  CAS  Google Scholar 

  • Wallenstein S, Zucker C, Fleiss J: Some statistical methods useful in circulation research. Circ. Res. 1980, 47, 1–9.

    Article  CAS  Google Scholar 

  • Vesanen M, Isomaa V, Alanko M, Vihko R: Cytosol estrogen and progesterone receptors in bovine endometrium after uterine involution postpartum and in the estrous cycle. Anim. Reprod. Sci. 1988, 17, 9–20.

    Article  CAS  Google Scholar 

  • Vesanen M, Isomaa V, Bolton N, Alanko M, Vihko R: Bovine steroid hormone and SHBG concentrations postpartum and during the oestrous cycle. Acta vet. scand. 1990, 31, 459–469.

    CAS  PubMed  Google Scholar 

  • Vesanen M, Isomaa V, Alanko M, Vihko R: Bovine uterine, cervical and ovarian estrogen and progesterone receptor concentrations. Anim. Reprod. Sci. 1991, 26, 61–71.

    Article  CAS  Google Scholar 

  • Vierikko P, Kauppila A, Vihko R: Cytosol and nuclear estrogen and progestin receptors and 17-beta-hydroxysteroid dehydrogenase activity in non-diseased tissue and in benign and malignant tumors of the human ovary. Int. J. Cancer 1983, 32, 413–422.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the Research Council for Medicine, and the Research Council for Agriculture and Forestry, of the Academy of Finland, the Oscar Öflund Foundation, the Finnish Veterinary Science Foundation and Coopers Information Center.

Author information

Authors and Affiliations

  1. Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland

    M. Vesanen

Authors
  1. M. Vesanen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vesanen, M. Bovine Uterine, Cervical and Ovarian Cytosol Estrogen and Progesterone Receptor Concentrations in Cystic Ovarian Disease. Acta Vet Scand 34, 35–43 (1993). https://doi.org/10.1186/BF03548221

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548221

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147