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Virulence, Cytotoxic and Inflammatory Activities of Vibrio Anguillarum and Aeromonas Salmonicida Isolated from Cultivated Salmonid Fish in Sweden

Virulens, cytoxisk och inflammatorisk aktivitet hos Vibrio anguillarum och Aeromonas salmonicida isolerad från odlad laxfisk i Sverige

Acta Veterinaria Scandinavica volume 28, pages 47–54 (1987)Cite this article

Abstract

Extracellular products in culture filtrates of Aeromonas salmonicida subsp. achromogenes and Vibrio anguillarum isolated from infected fish have been shown to possess skin inflammatory factor. The extracellular products from Vibrio anguillarum were cytotoxic in HeLa and CHO cells. In addition to the skin lesions, the culture filtrates of V. anguillarum caused necrotic reaction on the rabbit skin. Five of 6 strains of V. anguillarum were lethal to mice after intraperitoneal administration of 3×107 CFU. Only 1 strain of 5 A. salmonicida subsp. achromogenes produced extracellular products which elicited cytotoxic effects in the CHO cells. None of the A. salmonicida subsp. achromogenes strains were lethal to mice. The cytotoxins were inactivated when heated at 65°C for 30 min. The results indicate that the thermolabile exotoxins are non-enterotoxic since they failed to stimulate fluid accumulation in the rabbit ileal loop and did not cause elongation of the CHO cells. The rounding off of CHO cells, as well as of HeLa cells indicate that the exotoxins may play an important role in fish diseases.

Sammanfattning

Genom att använda olika biologiska tester kan man demonstrera hur Aeromonas salmonicida subsp, achromogenes och Vibrio anguillarum d.v.s. fiskpatogena bakterier producerar extraccllulära produkter. Dessa extracellulära produkter i bakteriella kulturfiltrat är cytotoxiska för HeLa och CHO cell linjer och orsakar inflammatoriska och nekrotiska reaktioner i kanin hud. Genom upphettning av kulturfiltrat till 65°C under 30 min inaktiveras dessa effekter. Fem av 6 Vibrio anguillarum stammar var lethala för möss efter intraperitoneal administration av 3×107 CFU. Ingen stam av Aeromonas salmonicida subsp. achromogenes var lethal för möss.

References

  • Austin B, Allen-Austin D: A review. Bacterial pathogens of fish. J. appl. Bact. 1985, 58, 483–506.

    Article  CAS  Google Scholar 

  • Bernheimer AW, Avigad LS: Partial characterization of aerolysin, lytic exotoxin from Aeromonas hydrophila. Infect. Immun. 1974, 9, 1016–1021.

    Article  CAS  Google Scholar 

  • Cipriano RC, Griffin BR, Lidgerding BC: Aeromonas salmonicida: Relationship between extracellular products and isolate virulence. Canadian J. Fish. Aquatic Sciences 1981, 38, 1322–1326.

    Article  CAS  Google Scholar 

  • Donta ST, Hadow AD: Cytotoxic activity of Aeromonas hydrophila. Infect. Immun, 1978, 21, 989–993.

    Article  CAS  Google Scholar 

  • Dubey RS, Sanyal SC: Enterotoxigeniçity of Aeromonas hydrophila: Skin responses and in vivo neutralization. Zbl. Bakt. Hyg., I. Abt. Orig. 1978, A242, 487–4199.

    Google Scholar 

  • Ellis AE, Hastings TS, Munro ALS: The role of Aeromonas salmonicida extracellular products in the pathology of furunculosis. J. Fish Dis. 1981, 4, 41–51.

    Article  Google Scholar 

  • Eurell TE, Lewis DH, Grumbles LC: Comparison of selected diagnostic tests for detection of motile Aeromonas septicaemia in fish. Amer. J. vet. Res. 1978, 39, 1384–1386.

    CAS  PubMed  Google Scholar 

  • Guerrant RL, Brunton LL, Schnaitman TC, Rebhun I, Gilman AG: Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: A rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect. Immun. 1974, 70, 320–327.

    Article  Google Scholar 

  • Huizinga HW, Esh GW, Hazen TC: Histopathology of red sore disease (Aeromonas hydrophila) in naturally and experimentally infected largemouth bass Micropterus salmoides. J. Fish Dis. 1979, 2, 263–277.

    Article  Google Scholar 

  • Hsu TC, Waltman WD, Shotts EB: Correlation of extracellular enzymatic activity and biochemical characteristics with regard to virulence of Aeromonas hydrophila. Develop. Biol. Stand. 1981, 49, 101–111.

    CAS  Google Scholar 

  • Janda JM, Clark RB, Brenden R: Virulence of Aeromonas species as assessed through mouse lethality studies. Curr. Mier. 1985, 72, 163–168.

    Google Scholar 

  • Janda JM, Bottone EJ, Skinner CV, Calcaterra D: Phenotypic markers associated with gastrointestinal Aeromonas hydrophila isolates from symptomatic children. J. clin. Mier. 1983, 17, 588–591.

    Article  CAS  Google Scholar 

  • Jiwa SFH: Enterotoxigeniçity, haemagglutination and cell-surface hydrophobicity in Aeromonas hydrophila, A. sobria and A. salmonicida. Vet. Microb. 1983, 8, 17–34.

    Article  CAS  Google Scholar 

  • McCarthy DH: Some ecological aspects of the bacterial fish pathogen Aeromonas salmonicida. Aqua Microb. Symposium No 6 of the Society for Applied Bacterol. 1977, 299–324.

    Google Scholar 

  • Sakai DK: Causative factors of Aeromonas salmonicida in salmonid furunculosis: extracellular protease. Scientific reports of the Hokkaido Fish Hatchery 1977, 32, 61–89.

    CAS  Google Scholar 

  • Sanyal SC, Singh SJ, Sen PC: Enteropathogenicity of Aeromonas hydrophila and Plesimonas shigelloides. J. med. Microb. 1975, 8, 195–199.

    Article  CAS  Google Scholar 

  • Sandefur PD, Peterson JW: Isolation of skin permeability factors from culture filtrates of salmonella typhimurium. Infect. Immun. 1976, 14, 671–679.

    Article  CAS  Google Scholar 

  • Shieh HS: Lethality of Aeromonas salmonicida to fish. Microb. Lett. 1981, 18, 29–32.

    Google Scholar 

Download references

Acknowledgements

We wish to express our sincere gratitude to Birgitta Evemar and Rakel Axelsson for the preparation of the manuscript.

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Authors and Affiliations

  1. The Department of Veterinary Microbiology, Section of Bacteriology and Epizootology, Swedish University of Agricultural Sciences and the National Veterinary Institute, Biomedicum, Box 583, S-75123, Uppsala, Sweden

    Karel Krovacek, Ahmed Faris, Laila Eriksson, Eva Jansson, Olle Ljungberg & Ingmar Månsson

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  1. Karel Krovacek
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  2. Ahmed Faris
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  4. Eva Jansson
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  5. Olle Ljungberg
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  6. Ingmar Månsson
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Krovacek, K., Faris, A., Eriksson, L. et al. Virulence, Cytotoxic and Inflammatory Activities of Vibrio Anguillarum and Aeromonas Salmonicida Isolated from Cultivated Salmonid Fish in Sweden. Acta Vet Scand 28, 47–54 (1987). https://doi.org/10.1186/BF03548255

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Acta Veterinaria Scandinavica

ISSN: 1751-0147