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Occurrence of Autoantibodies against Thyroglobulin in Norwegian Dogs
Forekomst av autoantistoffer mot thyrogtobulin hos norske hunder
Acta Veterinaria Scandinavica volume 28, pages 65–71 (1987)
Abstract
Serum samples from 45 hypothyroid dogs, 11 clinically healthy dogs and 31 dogs with various clinical endocrinopathies, seborrhoea and dermatitis were tested for thyroglobulin antibodies using the passive hemagglutination test. A chromic chloride (CCH)- and a glutaraldehyde (GCH) method were used in the assay. Circulating antibody titer against thyroglobulin were observed in 34 % and 24 % of dogs with hypothyroidism by the CCH and the GCH method, respectively. Three of 12 dogs with seborrhoea had low titers (20 to 40) of thyroglobulin antibodies. Thyroglobulin antibodies were observed in 1 of 3 hypothyroid dogs with thyroid carcinoma. Clinically healthy dogs and dogs with certain other endocrinopathies (Addison’s disease, Cushing’s disease, Diabetes mellitus, Hyperestrogenism) and dermatitis were negative.
Though the CCH method detected thyroglobulin antibodies in more serum samples than the GCH method, the GCH method was considered more reliable in that the glutaraldehyde treated cells were more stable, both at room temperature and at +4°C.
Sammendrag
Serumprøver fra 45 hunder med hypothyroidisme, 11 klinisk friske hunder og 31 hunder med forskjellige kliniske endokrinopatier, seborre og dermatitt ble undersøkt med hensyn til thyroglobulin antistoffer, ved å bruke passiv hem agglutinasjons test.
En kromklorid (CCH) metode og en glutaraldehyd (GCH) metode ble brukt i undersøkelsen. Sirkulerende antistoffer mot thyroglobulin ble påvist hos 34 % og 24 % av hundene med hypothyroidisme ved henholdsvis CCH og GCH metoden. Tre av 12 hunder med seborre hadde lave titre (20 og 40) av thyroglobulin antistoffer. Thyroglobulin antistoffer ble påvist hos I av 3 hypothyroide hunder med thyroideacarcinom.
Klinisk friske hunder og hunder med visse andre endokrinopatier (Addison's disease, Cushing's disease. Diabetes mellitus, Hyperøstrogenisme) og dermatitt var negative.
Selv om det ble påvist thyroglobulin antistoffer i flere serumprøver ved bruk av CCH metoden enn ved GCH metoden, ble GCH metoden betraktet som mer pålitelig siden glutaraldehyd behandlede celler var mer stabile både i rom temperatur og ved +4°C.
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Acknowledgement
This study was supported by a grant from the Norwegian Agricultural Research Council.
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Vollset, I., Larsen, H.J. Occurrence of Autoantibodies against Thyroglobulin in Norwegian Dogs. Acta Vet Scand 28, 65–71 (1987). https://doi.org/10.1186/BF03548257
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DOI: https://doi.org/10.1186/BF03548257