Growth of Preimplatation Bovine Embryos
Acta Veterinaria Scandinavica volume 35, pages 307–320 (1994)
Development of mammalian embryos in vitro is functionally and temporally inferior to embryo development as it occurs inside the female reproductive tract. The deficiencies of cultured embryos range from slow cleavage rates to complete developmental arrests or blocks, occurring at particular stages in many species. A variety of approaches have been used to overcome the blocks, including most extensively the coculture of preimplantation embryos with various somatic cells. However, even with coculture, development of embryos in vitro is still not equivalent to that in vivo. In most laboratories, only 25–40% of inseminated oocytes develop into morulae and blastocysts in spite of numerous variations on the basic technique. A better understanding of the factors governing embryonic growth is required before we can hope to achieve results comparable with those occurring in vivo.
In vitro utvikling av pattedyrembryo er både funksjonelt og tidsmessig dårligere enn embryo som utvikler seg naturlig i de hunnlige kjønnsveier. Manglene ved dyrkede embryoner varierer fra lav delingshastighet til fullstendig delingsstopp, som inntrer på bestemte stadier hos mange arter. Forskjellige metoder har blitt brukt for å forhindre denne såkalte blokkering, inklusive kultur av embryo med forskjellige somatiske celler. Til tross for bruk av slike systemer er in vitro utvikling av embryo forsatt ikke sammenlignbart med in vivo utvikling. Til tross for en rekke variasjoner i de benyttede teknikker flater nivået av utviklede morulae/blastocyster ved 25–40% av inseminerte oocytter i de fleste laboratorier. Det kreves en bedre forståelse av faktorene som styrer embryoets vekst før vi kan håpe å oppnå resultater som kan sammenlignes med forhold in vivo.
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Lonergan, P. Growth of Preimplatation Bovine Embryos. Acta Vet Scand 35, 307–320 (1994). https://doi.org/10.1186/BF03548303
- cell cycle
- in vitro