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Characterization of Vibrio anguillarum Strains Isolated from Diseased Fish in Finland

Karakterisering af Vibrio anguillarum isoleret fra sy ge fisk i Finland

Acta Veterinaria Scandinavica volume 35, pages 355–362 (1994)Cite this article

Abstract

Characterization of V anguillarum strains (n=109) isolated from diseased salmonids was performed. Eight O serovars were found among the strains. Serovar Ol was predominant (90 %), while serovars O2, O3, O5, O8, O9, and a new serovar Va NT2, were represented by 1 or 2 strains. Two strains remained non-typeable. One of these was cross-reactive with several antisera, but had a LPS profile identical to that of serovar O8. All serovars showed specific LPS profiles. All but 1 of the Ol strains had a plasmid comparable in size to the pJMl virulence plasmid, while plasmids of different sizes were found in O2, Va NT2 and the non-typeable strains. Apart from a single strain resistant to tetracycline, all the strains were sensitive to oxolinic acid, tetracycline, and trimethoprim-sulfonamides. By their biochemical and antigenic properties strains causing vibriosis among salmonids in Finland closely resemble Scandinavian strains. Predominance of the serovars Ol and O2 suggests that commercial vaccines containing these serovars should afford sufficient protection against vibriosis in Finland.

Sammendrag

I alt 109 V. anguillarum stammer isoleret fra salmonider blev karakteriseret. Stammerne var delt i 8 serovar, hvoraf serovar O1 var dominerende (90 %). Serovar O2, O3, O5, O8, O9 og en ny serovar Va NT2 var repræsenteret med enkelte stammer. To stammer kunne ikke types, en af dem krydsreagerede med flere antisera. Krydsreagerende stamme havde LPS-profil som lignede serovar 08 LPS-profil, ellers havde hver serovar en specifik LPS-profil. Alle O1 stammerne, undtagen én, havde et stort pJM1 virulens-plasmid lignende plasmid. Plasmider i forskellige størrelser blev fundet i O2, Va NT2 og i ikke typbare stammer. Bortset fra en enkelt stamme, som var resistent overfor tetracyclin, var alle stammerne sensitive for oxolinsyre, trimetoprim-sulfamethizol og tetracyklin. I deres biokemiske reaktioner lignede finske stammer skandinaviske stammer. At flertallet af de finske stammer tilhørte serovar O1 og O2 tyder på, at kommercielle vacciner anvendt i finsk akvakultur burde være effektive mod vibriose i Finland.

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Acknowledgments

We thank Raili Schildt, National Veterinary and Food Research Institute, Helsinki and Hille vi Niiranen, Ã…bo Akademy, Turku, for providing strains for this study, Karl Pedersen and Nancy Noben for critical reading of the manuscript and Tarja Pohjanvirta for helpful ideas during the study. This study was supported by the Foundation of the High Technology of Savo, Finland, and by the Nordic Council of Ministers, Nordic Programme Committee for Biotechnology.

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Authors and Affiliations

  1. Regional Laboratory of Kuopio, National Veterinary and Food Research Institute, P.O. Box 92, SF-70211, Kuopio, Finland

    T. Tiainen & S. Pelkonen

  2. Department of Veterinary Microbiology, Laboratory of Fish Diseases, Royal Veterinary and Agricultural University, Frederiksberg, Denmark

    J. L. Larsen

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  1. T. Tiainen
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  2. J. L. Larsen
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  3. S. Pelkonen
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Tiainen, T., Larsen, J.L. & Pelkonen, S. Characterization of Vibrio anguillarum Strains Isolated from Diseased Fish in Finland. Acta Vet Scand 35, 355–362 (1994). https://doi.org/10.1186/BF03548308

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Acta Veterinaria Scandinavica

ISSN: 1751-0147