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Freezability and Fertility Results with Uncentrifuged Stallion Semen

Spermiernas frysbarhet och dräktighetsresultat med ocentrifugerad hingstsperma

Acta Veterinaria Scandinavica volume 35, pages 377–382 (1994)Cite this article

Abstract

The first (1 to 3) sperm-rich fractions of the ejaculate were collected from 4 stallions using an open-ended vagina. The volume of the collected fractions was 12 ± 8 ml with a density of 475 ± 200 million spermatozoa/ml. Before freezing, the semen was diluted with a skim-milk based extender 1:1 to 1: 8 (volume of semen: volume of extender), depending on the initial sperm concentration to achieve a final concentration of 100 million/ml. The total number of spermatozoa in an insemination dose ranged from 0.7 to 1 billion spermatozoa. Within 12 h after ovulation, 48 mares were inseminated in 70 cycles. The total single-cycle pregnancy rate at day 21 was 24%, but varied from 10% to 33% per cycle among the stallions.

Sammanfattning

Sammanlagt 19 ejakulat samlades från 4 hingstar med öppen vagina. Totalvolymen av de 1, 2 eller 3 första spermierika fraktionerna var 12 ± 8 ml och koncentrationen 475 ± 200 milj. spermier/ml. Sperman späddes genast efter uppsamlingen med mjölkbaserad spädningsvätska och djupfrystes. Totalantalet spermier i de 7–9 ml inseminationsdoserna var 0.7 till 1 miljard och koncentrationen 100 milj. spermier/ml. Spädningsgraden varierade mellan 1:1 – 1:8 (sperma : spädningsvätska). Vid en follikelstorlek på 35 mm, kontrollerades stona 2 gånger dagligen både med palpation och ultraljud. Senast 12 timmar efter Ovulationen inseminerades 48 ston under sammanlagt 70 brunster. Dräktighetsresultatet (21 d) var 24% per inseminerad brunst. Mellan hingstarna varierade resultatet från 10% till 33%.

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Acknowledgments

The authors wish to thank Mrs. Marja Miskala, Ms. Arja Mansala, Ms. Helena Mäkelä, Mr. Niilo Lyytikäinen, Ms. Tiina Riihimäki, and Ms. Virpi Vuori for excellent technical assistance and Dr. Mir ja Huhtinen, Dr. Tiina Kotilainen, Dr. Helena Kuntsi, and Dr. Elina Kyynäräinen for their help during the insemination trials at the Equine Research Station. This work was supported in part by grants from the Research Council for Agricultural Sciences, the Academy of Finland.

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Authors and Affiliations

  1. Department of Biochemistry & Biotechnology, University of Kuopio, P.O. Box 1627, SF-70211, Kuopio, Finland

    M.-L. Heiskanen, A. Pirhonen & P H. Mäenpää

  2. Equine Research Station, Agricultural Research Centre, Ypäjä, Finland

    L. Hilden, S. Hyyppä & A. Kangasniemi

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  1. M.-L. Heiskanen
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  3. S. Hyyppä
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Heiskanen, ML., Hilden, L., Hyyppä, S. et al. Freezability and Fertility Results with Uncentrifuged Stallion Semen. Acta Vet Scand 35, 377–382 (1994). https://doi.org/10.1186/BF03548311

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Acta Veterinaria Scandinavica

ISSN: 1751-0147