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Hypophosphatemia Induced by Dietary Aluminium Hydroxide Supplementation in Growing Pigs: Effects on Erythrocytes, Myocardium, Skeletal Muscle and Liver

Acta Veterinaria Scandinavica volume 35, pages 263–271 (1994)Cite this article

Abstract

Three groups of pigs were studied during and after 10 weeks of treatment with either Al(OH)3 (Al[OH]3-group, n=8) to induce hypophosphatemia, A1P04 (AlP04-group, n=8, aluminium control without hypophosphatemia) or no addition to the feed (control group, n=8). Blood samples were taken at the start of the experiment and after 3, 6 and 10 weeks and were analyzed for phosphate, calcium and 2,3-diphosphoglycerate (2,3-DPG). Samples from myocardium, skeletal muscle and liver were obtained in connection with exsanguination and analyzed for glycogen, adenosine-tri-phosphate (ATP), creatine phosphate (CP), glucose-6-phosphate (G-6-P) and lactate. The Al(OH)3-group became hypophosphatemic and hypercalcémie with low levels of 2,3-DPG in erythrocytes within 3 weeks and showed a retarded growth rate. After 10 weeks the Al(OH)3-group had low levels of ATP in myocardium as compared with the control-group and low levels of G-6-P as compared with the AlP04-group. No disturbances on electro-cardiograms registered at rest could be documented. G-6-P concentration was low in the biceps muscle in the Al(OH)3-group as compared with the AlP04-group and in the liver low G-6-P concentration was seen in addition to high lactate concentration. The fibre type composition in M. Longissimus did not differ between groups, but the Al(OH)3-group had, due to retardation in growth, smaller mean fibre-areas than pigs in the AlP04-group. Hypophosphatemia gave rise to high serum calcium levels, low concentration of 2,3-DPG in erythrocytes and influenced G-6-P concentration in skeletal muscle, G-6-P and ATP in myocardium, G-6-P and lactate in liver. Retarded growth was one serious consequence of hypophosphatemia and the disturbed energy metabolism.

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Acknowledgments

This work was supported by grants from the Swedish Council for Forestry and Agricultural Research (SJFR), the Swedish Medical Research Council (nr 5992) and the Karolinska Institute.

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Authors and Affiliations

  1. Department of Medicine and Surgery, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden

    L. Håglin, B. Essén-Gustavsson & A. Lindholm

  2. Department of Social Medicine, University Hospital, S-901 85, Umeå, Sweden

    L. Håglin

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  1. L. Håglin
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  2. B. Essén-Gustavsson
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  3. A. Lindholm
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Håglin, L., Essén-Gustavsson, B. & Lindholm, A. Hypophosphatemia Induced by Dietary Aluminium Hydroxide Supplementation in Growing Pigs: Effects on Erythrocytes, Myocardium, Skeletal Muscle and Liver. Acta Vet Scand 35, 263–271 (1994). https://doi.org/10.1186/BF03548331

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Acta Veterinaria Scandinavica

ISSN: 1751-0147