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Follicullar Development and Embryo Recovery Following 3 versus 8 FSH Injections in Heifers
Acta Veterinaria Scandinavica volume 35, pages 89–92 (1994)
Abstract
Ovarian follicular dynamics and embryo yield were studied during 2 different FSH regimens for superovulation of cattle. Twenty heifers were given intramuscular injections of FSH (total of 35 mg NIH) either once daily for 3 days (Group 3×1) or twice daily for 4 days (Group 4×2). At 72 h after the first FSH injection, each animal was injected with 0.75 mg cloprostenol. Inseminations were performed at 12 h and 24 h after the onset of heat. Transrectal ultrasonography was performed on the day of the first FSH injection, the day of cloprostenol injection, the day of insemination and finally on the day of embryo recovery (day 6 or 7 after heat). The numbers of small (2–4 mm), medium (5–9 mm) and large (>10 mm) size follicles were recorded. The total number of corpora lutea, eggs and transferable embryos were recorded on the day of embryo recovery. No differences were found between the 2 groups in either of the parameters studied (p>0.05). It can be concluded that treatment with this FSH preparation once daily for 3 days gives a folliculogenic and superovulatory response similar to a treatment regimen where it is given twice daily for 4 days.
References
Bo GA, Pierson RA, Mapletoft RJ: The effect of estradiol valerate on follicular dynamics and superovulatory response in cows with Synchro-mate-B implant. Theriogenology, 1992, 36, (169–183).
Callesen H, Greve T, Hyttel P: Premature ovulations in superovulated cattle. Theriogenology, 1987, 28, (155–166).
Donaldson LE: Embryo production by Super-ov and FSH-P. Theriogenology, 1990, 33, 214 (abstr).
Donaldson LE: The efficacy of 3 superovulation regimens in cattle. Theriogenology, 1991, 35, 195 (abstr).
Greve T: Bovine egg transplantation in Denmark. Dr.vet.Sci. Thesis, Copenhagen, Denmark 1981. 221 pp.
Hill KG, Bondioli KR, Looney CR: Norgestomet incorporation into a superovulation regime. Theriogenology, 1986, 25, 160 (abstr.).
Hockley DK, Bo GA, Palasz AT, Del Campo MR, Mapletoft RJ: Superovulation with a single subcutaneous injection of Foiltropin in the cow: Effect of dose and site of injection. Theriogenology, 1992, 37, 224 (abstr.).
Laster DB: Disappearance and uptake of (125 I) FSH in the rat, rabbit, ewe and cow. J. Reprod. Fert., 1972, 30, (407–415).
Lindner GM, Wright RW Jr.: Bovine embryo morphology and evaluation. Theriogenology, 1983 20, 407–416.
Misra AK, Chaubal SA, Krisna Kishore G, Rajesh-waran S, Joshi BV, Jaiswal RS: Superovulatory response to single subcutaneous injection of Foiltropin in holstein and sahiwal cows. Theriogenology, 1992, 37, 260 (abstr.).
Monniaux D, Chupin D, Saumande J: Superovulatory response of cattle. Theriogenology, 1983, 19, (55–64).
Purwantara B, Schmidt M, Greve T, Callesen H: Follicular dynamics prior to and during superovulation in cattle. Theriogenology, 1993, 40, (913–21).
Schmidt M, Purwantara B, Callesen H, Klindt K, Greve T: The effect of 3 versus 8 injections with identical doses of Foiltropin Vet® on the embryo recovery in dairy cows. Proc. 8th Scientific Meeting of Europ. Emb. Transfer Assoc., Lyon, 1992, 210 (abstr.).
Yamamoto M, Suzuki T, OoeM, TagakiM, Kawaguchi M: Efficacy of single vs multiple injection superovulation regimens of FSH using polyvinylpyrrolidone. Theriogenology, 1992, 37, 325 (abstr.).
Acknowledgments
This study was supported by Animal Biotechnology Research Center and Danish Internatioal Development Agency (DANIDA).
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Purwantara, B., Schmidt, M., Callesen, H. et al. Follicullar Development and Embryo Recovery Following 3 versus 8 FSH Injections in Heifers. Acta Vet Scand 35, 89–92 (1994). https://doi.org/10.1186/BF03548358
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DOI: https://doi.org/10.1186/BF03548358