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Liberation of PGF from Whole Blood as an Assay to Detect Inflammatory Mediators

Abstract

The aim of this study was to evaluate a simple method of monitoring an inflammatory process based on the reaction of normal whole blood to plasma from animals suffering from an induced acute inflammation. There is a strong need to find relevant methods to be able to screen for mediators of inflammatory reactions thereby facilitating diagnosis of inflammatory processes. In e.g. induced endo-toxaemia, the endotoxin disappears very quickly from the circulation and is not a suitable substance to analyse in plasma (Aiumlamaietal 1990).

References

  • Aiumlamai S, Fredriksson G, Kindahi H, Edqvist L-E, Kulander L, Eriksson Ö: Endotoxin concentrations in the blood following intravenous injection and effect on prostaglandin F2a release, calcium and bile acids in goats. Res. vet. Sci. 1990, 48, (190–195).

    Article  CAS  Google Scholar 

  • Lindberg AA, Greisman SE, Svenson SB: Induction of endotoxin tolerance with nonpyrogenic O-antigenic oligosaccharide-protein conjugates. Infect. Immun. 1983, 41, (888–895).

    Article  CAS  Google Scholar 

  • Lindgren J-Å, Kindahi H, Hammarström S: Radioimmunoassay of prostaglandins E2 and F2a in cell culture media utilizing antisera against prostaglandins F2ß and F2a. FEBS Lett. 1974, 48, (22–25).

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by grants from the Swedish Council for Forestry and Agricultural Research.

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del Campo, B.Z., Kindahl, H. Liberation of PGF from Whole Blood as an Assay to Detect Inflammatory Mediators. Acta Vet Scand 35, 99–101 (1994). https://doi.org/10.1186/BF03548360

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  • DOI: https://doi.org/10.1186/BF03548360