Ochratoxin A as a Suppressor of Mitogen-Induced Blastogenesis of Porcine Blood Lymphocytes

Abstract

The in vitro effect of ochratoxin A on pig lymphocytes stimulated by the mitogen Concanavalin A was studied by measuring the rates of ³H-thymidine incorporation in DNA.

Ochratoxin A inhibited the mitogenic response to Concanavalin A in a dose dependent way. An almost total inhibition was obtained with ≥ 1 mg ochratoxin A/1, approximately 60 % inhibition was produced by 0.5 mg ochratoxin A/1 and approximately 10 % inhibition by 0.06 mg ochratoxin A/1.

The immunosuppressive effect of ochratoxin A was not altered much by different contents of bovine serum albumin, 0.1 or 0.3 %, in the cell culture medium.

Sammanfattning

Effekten in vitro av ochratoxin A på stimulering av grislymfocyter med mitogenet Concanavalin A studerades genom att mäta mängden ³H-thymidin som inkorporerades i lymfocyternas DNA. Ochratoxin A hämmade mitogeneffekten av Concanavalin A på ett dosrelaterat sätt. En nästan total inhibering erhölls med ≥ 1 mg ochratoxin A/1, ungefar 60% inhibering åstadkoms med 0,5 mg ochratoxin A/1 och ungefar 10% inhibering med 0,06 mg ochratoxin A/1.

Den immunosuppressiva effekten av ochratoxin A påverkades inte i nämnvärd omfattning av olika mängder av bovint serum albumin, 0,1 eller 0,3 %, i cellodlingsmediet.