- Published:
Histochemical Demonstration of the Inhibitory Effect of Nuvan® and Neguvon® on Cholinesterase Activity in Pseudodactylogyrus anguillae (Monogenea)
Histokemisk påvisning af den hæmmende effekt af Nuvan® og Neguvon® på cholinesterase aktivitet i Pseudodactylogyrus anguillae (Monogenea)
Acta Veterinaria Scandinavica volume 29, pages 51–55 (1988)
Abstract
The nervous system of Pseudodactylogyrus anguillae, a gill parasite from European eel (Anguilla anguilla) is demonstrated histochemically using the acetylthiocholine iodide method. It was shown that the staining of the nervous system was reduced or eliminated due to the inhibitory effect of Nuvan® and Neguvon®.
Sammendrag
Nervesystemet i den monogene gælleparasit P. anguillae fra Anguilla anguilla er påvist ved hjælp af den histokemiske metode acetylthiocholin-iodid-metoden. Det blev demonstreret, at in vitro behandling af P. anguillae med Neguvon og Nuvan hæmmede cholinesterase i parasitterne, så farvningen af nervesystemet forhindredes.
References
Buchmann K, Mellergaard S, Køie M: Pseudodacty-logyrus infections in eel: a reviw. Dis.aquat. Org. 1987, 5, 51–57.
Bueding E, Schiller E L, Bourgeois J G: Some physiological, biochemical and morphological effects of TRIS (p-aminophenyl) cabonium salts (TAC) on Schistosoma mansoni. Amer. J. trop. Med. Hyg. 1967, 16, 500–515.
Chan B, Wu B: Studies on the pathogenicity, biology and treatment of Pseudodactylogyrus for eels in fishfarms. Acta zool. sin. 1984, 30, 173–180.
Egusa S: Notes on the culture of the European eel (Anguilla anguilla L.) in Japanese eel-farming ponds. Rapp. P. v. Reun. Cons. int. Explor. Mer. 1979, 174, 51–58.
Goven BA, Gilbert J P, Gratzek JB: Apparent drug resistance to the organophosphate dimethyl (2,2,2-trichloro-l-hydroxyethyl) phosphonate by monogenetic trematodes. J. Wildl. Dis. 1980, 16, 343–446.
Gussev A V: New genus of monogenetic trematode from an eel of genus Anguilla. Trudy Zool. Inst. Leningrad. 1965, 35, 119–125.
Halton D W, Jennings J B: Demonstration of the nervous system in the monogenetic trematode Di-plozoon paradoxum Nordmann by the indoxyl acetate method for esterases. Nature 1964, 202, 510–511.
Halton D W, Jennings J B: Observations on the nutrition of monogenetic trematodes. Biol. Bull. Mar. Biol. Lab., Woods Hole. 1965, 129, 257–272.
Haiton D W, Morris G P: Occurrence of cholinesterase and ciliated sensory structures in a fish gill-fluke, Diclidophora merlangi (Trematoda: Mo-nogenea). Z. Parasitenk. 1969, 33, 21–30.
Imada R, Muroga K: Pseudodactylogyrus micror-chis (monogenea) on the gills of cultured eels -III. Experimental control by trichlorfon. Bull. Jap. Soc. Sci. Fish. 1979, 45, 25–29.
Jennings J B, LeFlore W B: The histochemical demonstration of certain aspects of cercarial morphology. Trans. Amer. Microsc. Soc. 1972, 91, 56–62.
Kikuchi H: Two new species of Japanese trematodes belonging to Gyrodactylidae. Annot. Zool. Jap. 1929, 12, 175–186.
Lyons KM: Sense organ of monogenean skin parasites ending in a typical cilium. Parasitology 1969, 59, 611–623.
Ogawa K, Egusa S: Studies on eel Pseudodactylogy-rosis - I. Morphology and classification of three dactylogyrids with a proposal of a new species, Pseudodactylogyrus microrchis. Bull. Jap. Soc. Sci. Fish. 1976, 42, 395–404.
Ramisz A, Szankowska Z: Studies on the nervous system of Fasciola hepatica and Dicrocoelium den-driticum by means of histochemical method for active acetylcholinesterase. Acta Parasit. Pol. 1970, 17, 217–223.
Ramisz A, Lamina J, Schoop G: Die Beeinflussung von Cholinesterase und Acetylcholinesterase durch das Phosphorsäureesterpräparat Tiguvon (Bayer) bei künstlich mit Trichinen infizierten Mäusen. (The influence upon Cholinesterase and acetylcholinesterase by a phosphoracid-ester-compound Tiguvon (Bayer) in mice infected with Trichinella spiralis). Zentbl. Bakt. Parasit. Kde. 1967, 204, 289–298.
Shaw M K: The fine structure of the brain of Gastro-cotyle trachuri (Monogenea: Platyhelminthes). Cell Tissue Res. 1982, 226, 449–460.
Tinsley R C, Wynne Owen R: Studies on the biology of Protopolystoma xenopodis (Monogenoidea): the oncomiracidium and life-cycle. Parasitology 1975, 71, 445–463.
Venkatanarsaiah J: Detection of Cholinesterase in the nervous system of the oncomiracidium of a monogenean, Pricea multae Ahauhan, 1945. Parasitology 1981, 82, 241–244.
Acknowledgements
The Nuvan® used in these experiments was kindly supplied by Ciba Geigy A/S.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Buchmann, K., Mellergaard, S. Histochemical Demonstration of the Inhibitory Effect of Nuvan® and Neguvon® on Cholinesterase Activity in Pseudodactylogyrus anguillae (Monogenea). Acta Vet Scand 29, 51–55 (1988). https://doi.org/10.1186/BF03548391
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03548391