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Deoxyribonuclease Inhibitory Activity in Pig Intestinal Contents
Hemningsaktivitet i tarminnhold hos gris overfor deoxyribonukleaser
Acta Veterinaria Scandinavica volume 18, pages 442–450 (1977)
Abstract
The inhibitory effect of pig intestinal contents on some microbial DNases and bovine pancreas DNase was examined employing an agar diffusion test. The molecular weight of 1 inhibitor as well as the electrophoretic patterns of the inhibitors were determined.
DNases from Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Clostridium perfringens and bovine pancreas were inhibited by intestinal contents. DNases from Clostridium septicum, Serratia marcescens, Proteus mirabilis, Aeromonas hydropnila and Pseudomonas aeruginosa were not inhibited. The concentrations of the inhibitory substances were considerably higher in contents from the small intestine than from the large intestine.
Using electrophoretic procedures on intestinal contents, 3 different fractions showing DNase inhibition were demonstrated. The molecular weight of one of the inhibitors was estimated to be about 30000.
Sammendrag
Den hemmende virkning av tarminnhold fra gris overfor enkelte mikrobielle DNaser og bovin pankreas DNase ble undersøkt ved å benytte en agar-diffusjonstest. Molekylvekten av en av inhibitorene samt inhibitorenes elektroforetiske mønster ble også bestemt.
DNaser fra Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Clostridium perfringens og bovin pankreas ble hemmet av tarminnhold, mens DNaser fra Clostridium septicum, Serratia marcescens, Proteus mirabilis, Aeromonas hydrophila og Pseudomonas aeruginosa ikke ble hemmet. Konsentrasjonen av de hemmende komponenter var vesentlig høyere i innhold fra tynntarmen enn fra tykktarmen.
Ved elektroforese påvistes 3 forskjellige fraksjoner som viste DNase-hemning. Molekylvekten til en av inhibitorene ble bestemt til ca. 30000.
References
Cooper E. J. M. L. Trautmann & M, Laskowski: Occurrence and distribution of an inhibitor for desoxyribonuclease in animal tissues. Proc. Soc. exp. Biol. (N.Y.) 1950, 73, 219–222.
Dabrowska W. E. J. Cooper & M. Laskowski: A specific inhibitor for desoxyribonuclease. J. biol. Chem. 1949, 177, 991–992.
Feinstein R. N.: Activation of the neutral deoxyribonuclease. J. biol. Chem. 1960, 235, 733–737.
Gupta S. & R. M. Herriott: Nucleases and their inhibitors in the cellular components of human blood. Arch. Biochem. 1963, 101, 88–95.
Herriott R. M. J. H. Connolly & S. Gupta: Blood nucleases and infectious viral nucleic acids. Nature (Lond.) 1961, 189, 817–820.
Lachica R. V. F. C. Genigeorgis & P. D. Hoeprich: Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 1971, 21, 585–587.
Lazarides E. & U. Lindberg: Actin is the naturally occurring, inhibitor of deoxyribonuclease I. Proc. nat. Acad. Sci. (Wash.) 1974, 71, 4742–4746.
Lindberg M. U.: Crystallization from calf spleen of two inhibitors of deoxyribonuclease I. J. biol. Chem. 1966, 241, 1246–1249.
Liven E.: The applicability of agar-diffusion test in measuring deoxyribonucleases in pig intestinal content. Acta vet. scand. 1976, 17, 244–254.
Liven E. & K. Staveland: Single cell protein in the diets of pigs and chickens. Effects on the activity of DNase in intestinal contents. Acta vet. scand. 1976, 17, 441–450.
Sandvik O.: Studies on casein precipitating enzymes of aerobic and facultatively anaerobic bacteria. Thesis. Veterinary College of Norway, Oslo 1962, 116 pp.
Sandvik O.: The occurrence of antibodies against staphylococcal deoxyribonucleases in blood sera from different species. Acta vet. scand. 1974, 15, 631–635.
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Liven, E. Deoxyribonuclease Inhibitory Activity in Pig Intestinal Contents. Acta Vet Scand 18, 442–450 (1977). https://doi.org/10.1186/BF03548407
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DOI: https://doi.org/10.1186/BF03548407