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Recovery of Infective 3Rd Stage Larvae of Haemonchus Contortus and Ostertagia Ostertagi Ry Migration in Agar Gel

Acta Veterinaria Scandinavica volume 18, pages 293–299 (1977)Cite this article

Abstract

A laboratory technique is described for the recovery of Haemonchus contortus and Ostertagia ostertagi infective larvae by migration in agar gel. The addition of bile increased the recovery rate of the haemonchus larvae, but had a somewhat depressive effect on the number of ostertagia larvae recovered. Similarly, storage at 4°G lowered the yield of larvae of both species, compared to freshly harvested larvae. However, the recovery rates for both species were sufficiently high to recommend the technique for isolation of the infective stages from field samples.

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Authors and Affiliations

  1. Parasitological Research Group, Institute of Veterinary Microbiology and Hygiene, Royal Veterinary and Agricultural University, Bulowsvej 13, DK-1870, Copenhagen V, Denmark

    William M. Mwegoha & Rolf Jess Jørgensen

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  1. William M. Mwegoha
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  2. Rolf Jess Jørgensen
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Mwegoha, W.M., Jørgensen, R.J. Recovery of Infective 3Rd Stage Larvae of Haemonchus Contortus and Ostertagia Ostertagi Ry Migration in Agar Gel. Acta Vet Scand 18, 293–299 (1977). https://doi.org/10.1186/BF03548426

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Acta Veterinaria Scandinavica

ISSN: 1751-0147