Skip to main content
Download PDF
  • Published:

Glutathione Peroxidase Activity in Porcine Blood

Acta Veterinaria Scandinavica volume 18, pages 323–334 (1977)Cite this article

Abstract

Determination of the seleno-enzyme glutathione peroxidase (GSH-Px) in blood from Danish Landrace pigs was done using a quantitative, spectrophotometric method and a simple “spot test”. A close correlation between the net reaction rate measured spectrophotometrically (Δ A/min.) and time for defluores-cence (minutes) was obtained (r2 = 0.72—0.77, P < 0.0005). From these results the factors used for a conversion of defluorescence time to u/g hemoglobin were evaluated. The results further showed that the “spot test” can be used as a screening method for detection of subnormal GSH-Px levels in pigs.

While red cell GSH-Px seems independent of the sex, an elevation of both plasma and red cell GSH-Px was found with increasing age of pigs. The normal range of red cell GSH-Px activity was wide, contrasting the small variations observed in the individual pig. Some evidence that porcine red cell GSH-Px is under genetical control was found and discussed in relation to the possible use of GSH-Px as an indicator of the pig's selenium status.

References

  • Allen, W. M., W. H. Parr, P. H. Anderson, Sylvia Berrett, B. Bradley & D. S. P. Patterson: Selenium and the activity of glutathione peroxidase in bovine erythrocytes. Vet. Rec. 1975, 96, 360–361.

    Article  CAS  PubMed  Google Scholar 

  • Berschneider, F.: Biochemische Untersuchungen zum Problem der Bewegungsanomalien des Fleischschweines. In Gesundheitliche Aspekte der Fleischschweinproduktion. (Biochemical investigations of anomalies of the locomotor system in pigs. In Health aspects of pig production), (ed. E. Wiesner), VEB Gustav Fischer Verlag, Jena 1972, p. 89–157.

    Google Scholar 

  • Board, P. G. & D. W. Peter: A simple test for glutathione peroxidase and selenium deficiency. Vet. Rec. 1976, 99, 144–145.

    Article  CAS  PubMed  Google Scholar 

  • Ewan, R. C.: Effect of selenium on glutathione peroxidase. J. Anim. Sci. 1976, 42, 1352.

    Google Scholar 

  • Ewan, R. C. & M. E. Wastell: Effect of vitamin E and selenium on blood composition of the young pig. J. Anim. Sci. 1970, 31, 343–352.

    Article  CAS  PubMed  Google Scholar 

  • Giinzler, W. A., H. Kremers & L. Flohe: An improved coupled test procedure for glutathione peroxidase (E.C.1.11.1.9) in blood. Z. klin. Chem. 1974, 10, 444–448.

    Google Scholar 

  • Hafeman, D. G., R. A. Sunde & W. G. Hoekstra: Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in rats. J. Nutr. 1974, 104, 580–587.

    Article  CAS  PubMed  Google Scholar 

  • Kubek, A., M. Hesselholt & P. B. Nielsen: Unders0gelser over plasma- arylesterasevariationer hos svin. (A study of plasma arylesterase variations in pig). Kgl. Vet.- og Landboh0jsk., Inst. Sterili- tetsforskn., Arsberetn. 1969, 12, 9–29. Kpbenhavn.

    Google Scholar 

  • Lawrence, R. A. & R. F. Burk: Species and tissue distribution of non selenium-dependent glutathione peroxidase activity. (Abstract) Fed. Prod. 1977, 36, 1095.

    Google Scholar 

  • Noguchi, T., A. H. Cantor & M. L. Scott: Mode of action of selenium and vitamin E in prevention of exudative diathesis in chicks. J. Nutr. 1973, 103, 1502–1511.

    Article  CAS  PubMed  Google Scholar 

  • Omage, S. T. & A. L. Tappel: Effect of dietary selenium on glutathione peroxidase in the chicks. J. Nutr. 1974, 104, 747–753.

    Article  Google Scholar 

  • Rotruck, J. T., A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman & W. G. Hoekstra: Selenium: biochemical role as a component of glutathione peroxidase. Science 1973, 179, 588–590.

    Article  CAS  PubMed  Google Scholar 

  • Simesen, M. G. & K. B. Pedersen: Selenium determinations in Danish swine affected with hepatosis dietetica. Acta vet. scand. 1975, 16, 137–139.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tappel, A. L.: Selenium-glutathione peroxidase and vitamin E. Amer. J. clin. Nutr. 1974, 27, 960–965.

    Article  CAS  Google Scholar 

  • Thompson, R. H., C. H. McMurray & W. J. Blanchflower: The levels of selenium and glutathione peroxidase activity in blood of sheep, cows and pigs. Res. Vet. Sci. 1976, 20, 229–231.

    Article  CAS  Google Scholar 

  • Trapp, A. L., K. K. Keahey, D. L. Whitenack & C. K. Whitehair: Vitamin E-selenium deficiency in swine: Differential diagnosis and nature of field problem. J. Amer. vet. med. Ass. 1970, 157, 289–300.

    CAS  PubMed  Google Scholar 

  • Van Kampen, E. J. & W. G. Zijlstra: Standardization of hemoglobino- metry. II. The hemoglobincyanide method. Clin. chim. Acta 1961, 6, 538–544.

    Article  PubMed  Google Scholar 

  • Van Vleet, J. F., W. Carlton & H. J. Olander: Hepatosis dietetica and mulberry heart disease associated with selenium deficiency in Indiana swine. J. Amer. vet. med. Ass. 1970, 157, 1208–1219.

    PubMed  Google Scholar 

  • Widar, J., M. Ansay & R. Hemset: Polymorphism of adenosine deaminase in the pig: allelic variation in erythrocytes. Anim. Blood Grps biochem. Genet. 1974, 5, 115–124.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Department of Physiology, Endocrinology and Bloodgrouping, Royal Veterinary and Agricultural University, Bulowsvej 13, DK-1870, Copenhagen V, Denmark

    P. Fogd Jørgensen, J. Hyldgaard-Jensen & J. Moustgaard

Authors
  1. P. Fogd Jørgensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Hyldgaard-Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Moustgaard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jørgensen, P.F., Hyldgaard-Jensen, J. & Moustgaard, J. Glutathione Peroxidase Activity in Porcine Blood. Acta Vet Scand 18, 323–334 (1977). https://doi.org/10.1186/BF03548430

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548430

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147