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Saponin Adjuvants. V. Precipitation Of Serum Components by Non-Purified Saponin Adjuvants in Agar Gel Diffusion.

Acta Veterinaria Scandinavica volume 18, pages 361–366 (1977)Cite this article

Abstract

The immunologically active adjuvant Quil A does not induce precipitating antibodies in rabbits. This was tested by immunodiffusion of serum samples taken after repeated injections of Quil A. Quil A does not react non-specifically with any of 6 different animal sera tested (rabbit, guinea pig, horse, sheep, cattle, and pig). Two commercially available saponins with known adjuvant activity (Saponin MT, E. Merck, and Saponin P 3, Food Industries) produced non-specific precipitation in double gel diffusion tests with all the sera, as did crude extracts of the South-American tree Quillaja saponaria Molina.}

This phenomenon in relation to the local tissue damage caused by non-purified saponin preparations is discussed.

References

  • Arstilla, P.: Characteristics of vesicular stomatitis virus envelopes released with saponin. J. gen. Virol. 1974, 24, 319–326.

    Article  Google Scholar 

  • Axelsen, N. H., J. Kroll & B. Weeke (Eds.): Quantitative immunoelectrophoresis. Scand. J. Immunol. 1973, 2, Suppl. 1.

    Google Scholar 

  • Basu, N. & B. P. Bastogi: Triterpenoid saponins and sapogenins. Phytochemistry 1967, 6, 1248–1270.

    Article  Google Scholar 

  • Charlier, G., B. Strobbe, J. Debecq & J. Leunen: Studies about the adjuvant activity of saponin fractions in foot-and-mouth disease vaccine. Arch. exp. Vet.-Med. 1973, 27, 783–789.

    CAS  Google Scholar 

  • Cho, C. T. & K. K. Feng: Non-immunological precipitation of serum by sodium dodecyl sulphate in agar diffusion. Appl. Microbiol. 1974, 28, 557–560.

    Article  CAS  Google Scholar 

  • Dalsgaard, K.: Thin-layer chromatographic fingerprinting of commercially available saponins. Dansk T. Farm. 1970, 44, 327–331.

    CAS  PubMed  Google Scholar 

  • Dalsgaard, K.: Saponin adjuvants. I. The presence of a nondialysable fraction of Quillaja saponaria Molina with adjuvant activity in foot-and-mouth disease vaccines. Bull. Off. int. Epiz. 1972, 77, 1289–1295.

    Google Scholar 

  • Dalsgaard, K.: Saponin adjuvants. III. Isolation of a substance from Quillaja saponaria Molina with adjuvant activity in foot-and-mouth disease vaccines. Arch. ges. Virusforsch. 1974, 44, 243–254.

    CAS  Google Scholar 

  • Ouchterlony, O.: Double diffusion in gel technique. Pro.gr. Allergy 1958, 5, 1–10.

    CAS  Google Scholar 

  • Schneider, L. G., M. Horzinek & R. Novicky: Isolation of a hemagglutinating, immunizing, and noninfectious subunit of the rabies virion. Arch. ges. Virusforsch. 1971, 34, 360–367.

    Article  CAS  Google Scholar 

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  1. The State Veterinary Institute for Virus Research, Lindholm, DK-4771, Kalvehave, Denmark

    K. Dalsgaard

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  1. K. Dalsgaard
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Dalsgaard, K. Saponin Adjuvants. V. Precipitation Of Serum Components by Non-Purified Saponin Adjuvants in Agar Gel Diffusion.. Acta Vet Scand 18, 361–366 (1977). https://doi.org/10.1186/BF03548433

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Acta Veterinaria Scandinavica

ISSN: 1751-0147