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Saponin Adjuvants. Vi. The Adjuvant Activity of Quil a in Trivalent Vaccination of Cattle and Guinea Pigs Against Foot-And-Mouth Disease
Acta Veterinaria Scandinavica volume 18, pages 367–373 (1977)
Abstract
The saponin adjuvant Quil A was investigated in trivalent vaccination against foot-and-mouth disease with a concentrated vaccine based on BHK suspension cell virus of the serotypes O, A and G. The activity in cattle was estimated on the basis of seroneutra-lizing antibodies. Five and 10 ml doses with or without 1 mg of Quil A were each injected into 6 animals. Seroneutralizing antibodies were estimated at regular intervals during a period of 29 weeks. The activity in guinea pigs was estimated by experimental challenge. One ml doses of serial 4-fold dilutions of the vaccine with or without 50 µg of Quil A were injected into 24 groups of 20 guinea pigs. Challenge was given 3 weeks after vaccination.
It was concluded that Quil A showed adjuvant activity in cattle and guinea pigs with all the serotypes used in the trivalent vaccination.
References
Croxton, F. E.: Elementary Statistics, with Applications in Medicine and the Biological Sciences. Dover Publications Inc. New York 1959.
Dalsgaard, K.: Saponin adjuvants. III. Isolation of a substance from Quillaja saponaria Molina with adjuvant activity in foot-and- mouth disease vaccine. Arch. ges. Virusforsch. 1974, 44, 243–254.
Dalsgaard, K., Merethe H. Jensen & K. J. Sorensen: Saponin adjuvants. IV. Evaluation of the adjuvant Quil A in the vaccination of cattle against foot-and-mouth disease. Acta vet. scand. 1977, 18, 349–360.
Jensen, Merethe H. & F. O. Sorensen: Concentration and cold storage of BHK 21 clone 13 suspension cells used in the large-scale production of FMD virus. Bull. Off. int. Epiz. 1975, 83, 399–406.
Karber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. (Contribution to the treatment of pharmacological data). Naunyn-Schmiedeberg’s Arch. exp. Path. Phar- mak. 1931, 162, 480–483.
Lei, J. C.: Application of polyethylene glycol in the preparation of a concentrated, purified foot-and-mouth disease vaccine. Present status for research. Bull. Off. int. Epiz. 1974, 81, 1169–1199.
Acknowledgment
The challenge experiments in guinea pigs and the vaccination of cattle were performed in the department of serology. Head: Dr. K. Schjerning-Thiesen.
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Dalsgaard, K., Jensen, M.H. Saponin Adjuvants. Vi. The Adjuvant Activity of Quil a in Trivalent Vaccination of Cattle and Guinea Pigs Against Foot-And-Mouth Disease. Acta Vet Scand 18, 367–373 (1977). https://doi.org/10.1186/BF03548434
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DOI: https://doi.org/10.1186/BF03548434