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Ontogenetic Development of Creatine Phosphokinase in Skeletal Muscles and Heart from Pigs
Ontogenetisk udvikling af kreatinfosfokinase i tvaerstribede muskier og hjerte hos svin
Acta Veterinaria Scandinavica volume 18, pages 143–151 (1977)
Abstract
Creatine Phosphokinase (CPK) in striated muscles shows only small changes in activity before birth. After birth and during the first month of extrauterine life the activity increases rapidly. The largest increase is seen in muscles with a glycolytic energy metabolism (m. long, dorsi) and the smallest in muscles with an oxydative energy metabolism (m. flexor dig. ped. sup.). The differences between these groups of muscles are statistically significant. In heart tissue the increase in CPK activity is lower, the levels amounting to 40 to 47 % of those in striated muscles.
Early in fetal life only the BB isoenzyme is found in striated muscles. Synthesis of M subunits of GPK starts between day 76 and 65 before birth and increases rapidly after this time leading to disappearance of the BB isoenzyme 24 days prior to birth and of the MB isoenzyme at birth. In muscles with an oxydative as well as in muscles with a glycolytic metabolism all GPK activity after birth is caused by the MM isoenzyme.
All three isoenzymes are present in heart tissue at the earliest prenatal stage investigated, the pattern being dominated by the BB isoenzyme. During further differentiation the MM isoenzyme increases and the BB isoenzyme decreases. The development is completed during the first month after birth with a final isoenzyme composition of 81 % MM and 19 % MB isoenzyme. kw|Keywords|k]pigs; k]ontogenesis; k]creatine phosphokinase; k]activity; k]isoenzymes
Sammendrag
CPK aktiviteten i tvaerstribede muskier hos svin er kun under- kastet små aendringer før fødslen. Ved overgangen til det ekstra- uterine liv indtræder en stigning, der fortsætter i den første mined efter fødslen. Stigningen er størst i muskier med et glykolytisk stof- skifte og mindst i muskier med et oxydativt stofskifte. Dette medfører et signifikant lavere indhold af CPK i oxydative muskier. I hjertevæv er stigningen i CPK aktiviteten mindre end i tværstribede muskier resulterende i et indhold af GPK på 40 til 47 % af indholdet i tværstribede muskier.
I de tidligste unders0gte fosterstadier findes kun BB isoenzymet i tværstribede muskier. Syntesen af M subunits begynder mellem 76 og 65 dage før fødslen og stiger markant herefter, siledes at BB isoenzymet ikke er til stede 24 dage før fødslen, og MB isoenzymet for- svinder ved fødslen. Dette medfører, at GPK aktiviteten i såvel oxydative som glykolytiske muskier efter fødslen udelukkende udgøres af MM isoenzymet.
Alle tre isoenzymer er til stede i hjertevæv i det tidligste under- søgte stadium med BB isoenzymet som det dominerende. Under den videre udvikling stiger indholdet af MM isoenzymet, samtidig med at BB isoenzymet falder. I løbet af den første mined efter fødslen afsluttes isoenzymændringerne, siledes at den endelige isoenzymsam- mensætning af hjertevæv udg0res af 81 % MM og 19 % MB.
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Acknowledgments
The authors wish to express their gratitude to Professor, dr. med. vet. J. Moustgaard on whose initiative the present investigation was undertaken.
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Cepica, S., Jørgensen, P.F. Ontogenetic Development of Creatine Phosphokinase in Skeletal Muscles and Heart from Pigs. Acta Vet Scand 18, 143–151 (1977). https://doi.org/10.1186/BF03548442
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DOI: https://doi.org/10.1186/BF03548442