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The Biochemical Activity of Enterotoxin and Non-Enterotoxin Producing Staphylococci

Năgra biokemiska egenskaper hos enterotoxinbildaride och icke enterotoxinbildande stafylokocker

Acta Veterinaria Scandinavica volume 18, pages 266–273 (1977)Cite this article

Abstract

One hundred and sixty-nine staphylococcal strains of human origin have been tested for production of enterotoxin A, B or Ci, coagulase activity, DNase activity, typical growth on ETGP-agar, hemolysin production and the breakdown of mannitol under aerobic conditions. Very good correlation was observed between enterotoxin production and coagulase activity, in that 82 % of the enterotoxin producing strains also synthesized coagulase. The correlation between DNase activity and positive reaction in mannitol to enterotoxin production was also good (80 % of the enterotoxic strains produced both DNase and aerobic acid from mannitol). Of the enterotoxin producing strains 66 % hemolysed bovine erythrocytes and 61 % were ETGP-positive. However, the frequency of hemolysing respectively ETGP-positive but non-enterotoxin producing strains was very high, viz. 46 % respectively 32 %. It is concluded that enterotoxin production can not to a satisfactory degree of security be predicted by means of the other biochemical characters.

Sammanfattning

169 stafylokockstammar isolerade från näsa, svalg och sår påmänniska har undersökts avseende Produktion av enterotoxin (A, B, G1), koagulas, DNas och hemolysin, typisk växt på ETGP-agar samt mannitoloxidationsförmåga. 82 % av de enterotoxinproducerande stammarna var koagulaspositiva, 80 % spjälkade DNA, 80 % oxiderade mannitol, 66 % hemolyserade bovina erytrocyter och 61 % var ETGP- positiva. Av de icke enterotoxinbildande stammarna var 46 % hemo- lyserande, 32 % ETGP-positiva, 24 % mannitolpositiva, 20 % DNas- positiva och 18 % koagulaspositiva.

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Acknowledgments

The authors wish to thank Mrs. Margareta Lagerberg and Mrs. Lilly Stridsberg for their skilful technical assistance.

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Authors and Affiliations

  1. Department of Food Hygiene, Royal Veterinary College, Roslagsvägen 101, S-104 05, Stockholm 50, Sweden

    Marie-Louise Danielsson & Bo Hellberg

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  1. Marie-Louise Danielsson
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  2. Bo Hellberg
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Danielsson, ML., Hellberg, B. The Biochemical Activity of Enterotoxin and Non-Enterotoxin Producing Staphylococci. Acta Vet Scand 18, 266–273 (1977). https://doi.org/10.1186/BF03548455

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Acta Veterinaria Scandinavica

ISSN: 1751-0147