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Progesterone and 15-Keto-13,14-Dihydroprostaglandin F2α Levels in Peripheral Circulation After Intrauterine Iodine Infusions in Cows

Progesteron och 15-keto-13,14-dihydroprostaglandin F2α nivåer i den perifera blodcirkulationen hos kor efter intrauterin jodtillförsel

Acta Veterinaria Scandinavica volume 18, pages 274–286 (1977)Cite this article

Abstract

The effect of intrauterine iodine infusion on estrous cycle length was studied in four cows. The infusions were performed at various times of the estrous cycle: early, middle, late, and during luteolysis. Blood samples were drawn every third hour from the jugular vein. Progesterone and 15-keto-13,14-dihydroprostaglandin F2α (the main metabolite of PGF2α) were measured to monitor luteal activity and prostaglandin release. No release of prostaglandins was observed immediately following intrauterine infusion. Infusion in two cows on day 5 of the estrous cycle resulted in prostaglandin release after 54 and 69 hrs., respectively, followed by luteal regression and the occurrence of estrus at approx. five days after infusion. Infusions performed on days 11 or 12 resulted in prostaglandin release after 147 and 120 hrs., respectively, followed by luteolysis and heat after a 19 day estrous cycle. Infusion in two cows at days 16 and 17 resulted in prostaglandin release after 117 hrs. in both animals. One cycle was prolonged whereas the other cycle was normal in duration. One cow infused on day 20 following the occurrence of the first prostaglandin surge had a cycle length of 26 days, whereas another cow infused on day 20 was not affected because luteolysis was essentially complete by the time of infusion. One animal infused on day 5 did not respond to the iodine infusion. In this animal, however, the corpus luteum was not completely developed prior to the infusion.

From this study it can be concluded: 1) intrauterine iodine infusions performed after the development of a progesterone secreting corpus luteum result in prostaglandin release within three to six days with the subsequent occurrence of luteolysis; 2) luteolysis wras in all cases observed in connection with prostaglandin F2α release of the same order of magnitude and duration as during normal luteolysis. kw|Keywords|k]prostaglandin release; k]progesterone; k]cow; k]es trous cycle; k]iodine infusion

Sammanfattning

Effekten av intrauterin jodtillförsel (jodsköljning) på östralcykel- längden har undersökts på fyra SRB-kor. Jodinfusioner gavs tidigt, mitt i och sent i östralcykeln samt även under luteolys. Blodprov ut- togs från vena jugularis en gång var 3:e timma. Blodplasmanivåerna av progesteron och 15-keto-13,14-dihydroprostaglandin F2α (huvud- metabolit till prostaglandin F2α) bestämdes för att avgöra lutealakti- vitet respektive prostaglandinfrisättning. Ingen frisättning av prostaglandin erhölls i direkt anslutning till jodinfusionen. Intrauterin jodtillförsel till två kor på dag 5 av östralcykeln resulterade i prostaglan-dinfrisättning 54 och 69 timmar senare, vilket orsakade luteolys och brunst 5 dagar efter infusionen. Jodtillförsel på dag 11 eller 12 av östralcykeln resulterade i prostaglandinfrisättning 147 respektive 120 timmar senare, och efterföljande brunst efter en 19-dagars östral- cykel. Intrauterin jodtillförsel på dag 16 och 17 av östralcykeln med- förde prostaglandinfrisättning 117 timmar senare hos båda djuren. Hos den ena kon var östralcykellängden normal medan den andra hade en förlängd östralcykel. En ko infunderades på dag 20 i östralcykeln just efter det att den normala prostaglandinfrisättningen star- tat, och denna frisättning blockerades tillfälligt så att östralcykeln förlängdes till 26 dagar. Ytterligare en ko erhöh intrauterin jodtillförsel på 20 :e dagen av östralcykeln, dock var luteolysen i det när-maste fullständig vid infusionstillfället, och någon förlängning av östralcykeln erhölls inte. En ko som infunderades på dag 5 i östral- cykeln uppvisade normal östralcykellängd sannolikt beroende på att corpus luteum var ofullständigt utvecklad vid infusionstillfället. Från föreliggande undersökning kan det konkluderas: 1) intrauterin jod- tillförsel given efter det att en progesteronproducerande corpus luteum har utvecklats resulterar i prostaglandinfrisättning och luteo- lys inom 3 till 6 dagar; 2) den på så sätt inducerade luteolysen obser- verades i samtliga fall samtidigt med prostaglandinfrisättningen, som till sin storlek och duration liknar den vid spontan luteolys.

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Acknowledgments

The authors are indebted to Miss Ulla Nordin and Mrs. Anette Häggström for expert technical assistance.

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Author notes

  1. Börje Gustafsson

    Present address: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA

Authors and Affiliations

  1. Department of Chemistry, Karolinska Instituted Stockholm, and the Department of Clinical Chemistry and the Department of Obstetrics and Gynaecology, Royal Veterinary College, S-750 07, Uppsala, Sweden

    Hans Kindahl, Elisabeth Granström, Lars-Eric Edquist, Börje Gustafsson, Göran Äström & George Stabenfeldt

  2. Department of Reproduction, School of Veterinary Medicine, University of California, Davis, USA

    George Stabenfeldt

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  1. Hans Kindahl
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Kindahl, H., Granström, E., Edquist, LE. et al. Progesterone and 15-Keto-13,14-Dihydroprostaglandin F2α Levels in Peripheral Circulation After Intrauterine Iodine Infusions in Cows. Acta Vet Scand 18, 274–286 (1977). https://doi.org/10.1186/BF03548456

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Acta Veterinaria Scandinavica

ISSN: 1751-0147