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A Simple Method for Determination of Pepsinogen in Bovine Serum and Plasma

Acta Veterinaria Scandinavica volume 18, pages 10–14 (1977)Cite this article

Abstract

Calf serum pepsinogen can be determined by radial diffusion in a casein-containiing agarose gel prepared at pH 8.2 and afterwards immersed in a BaGla-containing buffer, pH 2.4, whereby the casein is precipitated homogeneously. Serum samples are placed in wells in the gel, and at the low pH pepsinogen present in the samples will be activated and the casein digested, whereby transparent, circular zones are formed in the otherwise opaque gel. The diameter of these zones can be measured directly and used as an expression of the serum pepsinogen level. On analysis of a number of sera from calves with or without ostertagiasis a positive correlation was found between the results obtained by this method and conventional absorption photometry (r = 0.87, n = 55).

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Acknowledgments

The author is indebted to Drs. R. Jess Jϕrgensen and P. Nansen, the Royal Veterinary and Agricultural University, Copenhagen, for their kindness in submitting sera already analyzed by the photometric method. Thanks are also due to Mrs. B. Lüders Jensen and Mrs. Vibeke S0rensen for the very skilful technical assistance.

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Authors and Affiliations

  1. The State Veterinary Serum Laboratory, Bülowsvej 27, DK-1870, Copenhagen V, Denmark

    P. Thode Jensen

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  1. P. Thode Jensen
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Jensen, P.T. A Simple Method for Determination of Pepsinogen in Bovine Serum and Plasma. Acta Vet Scand 18, 10–14 (1977). https://doi.org/10.1186/BF03548460

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  • DOI: https://doi.org/10.1186/BF03548460

Acta Veterinaria Scandinavica

ISSN: 1751-0147