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A Study of Phage- and Ribotype Patterns of Staphylococcus aureus Isolated from Bovine Mastitis in the Nordic Countries

Acta Veterinaria Scandinavica volume 38, pages 243–252 (1997)Cite this article

Abstract

This study was conducted to investigate the geographical distribution of phage and ribotypes of Staphylococcus aureus causing bovine mastitis in the 5 Nordic countries. A total of 403 isolates of S. aureus was isolated from 403 different dairy herds. One hundred five strains were isolated in Denmark, 81 in Finland, 17 in Iceland, 96 in Norway and 104 in Sweden. The isolates were phage typed and characterized for their EcoRI restriction fragment length polymorphisms of the genes encoding ribosomal RNA (ribotyping). A total of 351 (87%) of the 403 isolates could be typed by phages assigning them to 25 different phage types. Two to 3 different phage types predominated within each country. One type (29/52) accounted for 36% of all the isolates and was found in 4 of the countries. A total of 87 different ribotypes was found among the isolates investigated. As for phage typing 2 to 3 different types predominated within countries. However, except for one type (ribotype 1), which was commonly found in Denmark, Sweden and Finland, different ribotypes predominated within each country. The combination of phage and ribotyping assigned the isolates to 178 different types. Ninety-six percent of the isolates of ribotype 1 belonged to phage type 29/52. This combined type accounted for 17% of all the 403 isolates. These findings show that a large number of different types of S. aureus can be isolated from cases of bovine mastitis. However, few types predominate within different countries. These predominating types seem to be specific in each country, however, a single type was common for both Denmark, Sweden and Finland. This could suggest differences in the virulence or in modes of transmission of predominating and rare types of S. aureus associated with bovine mastitis.

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Acknowledgment

We are grateful to Rene Hendriksen for technical assistance. This work was supported by a grant from the Danish Ministry of Agriculture (SUN 93-5).

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Authors and Affiliations

  1. Danish Veterinary Laboratory, Bülowsvej 27, DK-1790, Copenhagen V, Denmark

    F.M. Aarestrup, H. C. Wegener & N. E. Jensen

  2. Mjolkursamlag KEA, Akureyri, Iceland

    O. Jonsson

  3. National Veterinary and Food Research Institute, Helsinki, Finland

    V. Myllys

  4. Swedish University of Agricultural Sciences, Uppsala, Sweden

    B. M. Thorberg

  5. Central Veterinary Laboratory, Oslo, Norway

    S. Waage

  6. Statens Serum Institut, Copenhagen, Denmark

    V. Thamdrup Rosdahl

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  1. F.M. Aarestrup
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  2. H. C. Wegener
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  3. N. E. Jensen
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  4. O. Jonsson
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  5. V. Myllys
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  6. B. M. Thorberg
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  7. S. Waage
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  8. V. Thamdrup Rosdahl
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Correspondence to F.M. Aarestrup.

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Aarestrup, F., Wegener, H.C., Jensen, N.E. et al. A Study of Phage- and Ribotype Patterns of Staphylococcus aureus Isolated from Bovine Mastitis in the Nordic Countries. Acta Vet Scand 38, 243–252 (1997). https://doi.org/10.1186/BF03548487

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Acta Veterinaria Scandinavica

ISSN: 1751-0147