Skip to main content
Download PDF
  • Published:

Serum enzyme determination in the study of liver disease in dogs

Serumenzymbestemmelser ved studium av leversykdom hos hunder

Acta Veterinaria Scandinavica volume 27, pages 59–70 (1986)Cite this article

Abstract

Glutamate dehydrogenase (GLDH), sorbitol dehydrogenase (SDH), 5'-nucIeotidase (5'-ND) and cholin esterase (CHE) were determined in the sera of 37 dogs with various liver diseases. The values for these parameters were compared with the values for aspartate and alanin aminotransferase (ASAT, ALAT), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT) and albumin (ALB). GLDH was found to be the most sensitive enzyme parameter (sensitivity 92 %), its values also reflecting the degree of liver cell necrosis well. Next in sensitivity followed ASAT and ALP. SDH values were correlated to ALAT values but had a low sensitivity (43 %). 5'-ND discriminated markedly better between biliary tract disturbance and other lesions than ALP and somewhat better than γ-GT. CHE had a wide reference range, low sensitivity, and was not correlated to ALB. Single determinations of CHE may thus not be of diagnostic value in dogs.

Sammendrag

Glutamatdehydrogenase (GLDH), sorbitoldehydrogenase (SDH), 5'-nukleotidase (5'-ND) og cholinesterase (CHE) ble bestemt i sera fra 37 hunder med forskjellige leversykdommer. Verdiene for disse parametrene ble sammenliknet med verdiene for aspartat- og alaninaminotransferase (ASAT, ALAT) alkalisk fosfatase (ALP), γ-glutamyl-transferase (γ-GT) og albumin (ALB). GLDH ble funnet å være den mest sensitive enzymparameter (sensitivitet 92 %) og GLDH-verdiene var samtidig et godt mål for graden av levercellenekrose. I sensitivitet fulgte dernest ASAT og ALP. SDH-verdiene var korrelert til ALAT-verdiene, men hadde lav sensitivitet (43 %). 5′-ND skilte vesentlig bedre mellom hepatobiliære og hepatocellulære skader enn ALP, og noe bedre enn γ-GT. GHE hadde et bredt referanseområde (normalomåde), lav sensitivitet og var ikke korrelert til ALB. Enkeltbestemmelser av CHE er derfor trolig uten diagnostisk betydning for hund.

References

  • Arkesteijn, C. L. M.: A kinetic method for serum 5’-nucleotidase using stabilized glutamate dehydrogenase. J. clin. Chem. clin. Bio- chem. 1976, 14, 155–188.

    CAS  Google Scholar 

  • Boyd, J. W.: The comparative activity of some enzymes in sheep, cattle and rats. Normal serum and tissue levels and changes during experimental liver necrosis. Rets. Vet. Sci. 1962, 3, 256–268.

    Google Scholar 

  • Cornelius, C. E., J. Bishop, J. Switzer & E. A. Rhode: Serum and tissue transaminase activities in domestic animals. Cornell Vet. 1959, 40, 116–126.

    Google Scholar 

  • Cornelius, C. E.: Biochemical evaluation of hepatic function in dogs. J. Amer. Anim. Hosp. Ass. 1979, 15, 259–269.

    CAS  Google Scholar 

  • De Broe, M. E., M. Borgers & R. J. Wieme: The separation and characterization of liver plasma membrane fragments circulating in the blood of patients with cholestasis. Clin. Chim. Acta 1975, 59, 369–372.

    Article  Google Scholar 

  • Doumas, B. T., W. A. Watson & H. G. Biggs: Albumin standards and the measurement of serum albumin with bromcresol green. Clin. Chim. Acta 1971, 31, 87–96.

    Article  CAS  Google Scholar 

  • Hauge, J. G. & S. V. Abdelkader: Serum bile acids as an indicator of liver diseases in dogs. Acta vet. scand. 1984, 25, 495–503.

    Article  CAS  Google Scholar 

  • Hoe, C. M. & J. D. O’Shea: The correlation of biochemistry and histopathology in liver disease in the dog. Vet. Rec. 1965, 77, 1164–1171.

    Article  CAS  Google Scholar 

  • Keller, P.: Enzyme activities in the dog: tissue analyses, plasma values, and intracellular distribution. Amer. J. vet. Res. 1981, 42, 575–582.

    CAS  Google Scholar 

  • McConnel, M. F. & J. H. Lumsden: Biochemical evaluation of metastatic liver disease in the dog. J. Amer. Anim. Hosp. Ass. 1983, 19, 173–178.

    Google Scholar 

  • Noonan, N. E. & D. J. Meyer: Use of plasma arginase and y-glutamyl transpeptidase as specific indicators of hepatocellular or hepatobiliary disease in the dog. Amer. J. vet. Res. 1979, 40, 942–947.

    CAS  Google Scholar 

  • Palm, M., I. Jones & L. E. Edquist: GT-aktiviteten i blod. En indikator for leverskada hos husdjur. (GT activity in blood: An indicator of liver damage in domestic animals). Svensk vet. tidning 1982, 34, 97–100.

    Google Scholar 

  • Rappaport, F., J. Fischl & N. Pinto: An improved method for the estimation of cholinesterase activity in serum. Clin. chem. Acta 1959, 4, 227–230.

    Article  CAS  Google Scholar 

  • Rogers, W. A. & B. H. Ruebner: A retrospective study of probable glucocorticoid-induced hepatopathy in dogs. J. Amer. vet. med. Ass. 1977, 170, 603–606.

    CAS  PubMed  Google Scholar 

  • Schall, W. D.: Laboratory diagnosis of hepatic disease. Vet. Clin. No. Amer. 1976, 6, 679–686.

    Article  CAS  Google Scholar 

  • Shull, R. M. & W. Hornbuckle: Diagnostic use of serum y-glutamyl- transferase in canine liver disease. Amer. J. vet. Res. 1979, 40, 1321–1324.

    CAS  Google Scholar 

  • Strombeck, D.R.: Glinicopathologic features of primary and metastatic neoplastic disease of the liver in dogs. J. Amer. vet. med. Ass. 1978, 173, 267–269.

    CAS  PubMed  Google Scholar 

  • Vorhaus, L. J. & R. M. Kark: Serum cholinesterase in health and disease. Amer. J. Med. 1953, 14, 707–719.

    Article  CAS  Google Scholar 

  • Zinkl, J. G., R. M. Bush, C. E. Cornelius & R. A. Freedland: Comparative studies on plasma and tissue sorbitol, glutamic, lactic and hydroxbutyric dehydrogenase and transaminase activities in the dog. Res. Vet. Sei. 1971, 12, 211–214.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to express their gratitude to the scientific and technical staff of the Department of Pathology for providing the desired histological sections and assisting in the interpretation of these.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Norwegian College of Veterinary Medicine, P. O. Box 8146, N-0033, Oslo 1, Norway

    Jens Gabriel Hange & Signe Videm Abdelkader

Authors
  1. Jens Gabriel Hange
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Signe Videm Abdelkader
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hange, J.G., Abdelkader, S.V. Serum enzyme determination in the study of liver disease in dogs. Acta Vet Scand 27, 59–70 (1986). https://doi.org/10.1186/BF03548559

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548559

Acta Veterinaria Scandinavica

ISSN: 1751-0147