Skip to main content
Download PDF
  • Published:

Rapid immunoassay for detection of Crypto-sporidium oocysts

En snabb immunmetod för påvisning av Cryptosporidium oocystor

Acta Veterinaria Scandinavica volume 27, pages 71–79 (1986)Cite this article

Abstract

A direct immunological method by means of latex agglutination (LX) reaction was applied to the demonstration of Cryptosporidium oocysts from stools and gut homogenates. The LX method gave a positive diagnosis for all the specimens judged positive by the modified Ziehl-Neelsen technique, which served as a reference method, The results of the study indicate that the LX method can be adopted as a diagnostic tool for cryptosporidiosis, but further study concerning the specificity and sensitivity of the method is clearly warranted.

Sammendrag

En direkt immunologisk metod baserad på latex agglutinations (LX) reaktion tillämpades för att påvisa Cryptosporidium oocystor i avföring och tarmhomogen at. LX metoden gav positiv diagnos för alla preparat som också var positiva med modifierad Ziehl-Neelsen teknik, vilken fungerade som referensmetod. Resultaten visar att LX metoden kan användas vid diagnostik av kryptosporidios, men ytterligare studier med avseende på metodens specifitet och sensitivitet är nödvändiga.

References

  • Anderson, B. C., T. Donndelinger, R. M. Wilkins & J. Smith: Cryptosporidiosis in a veterinary student. J. Amer. vet. med. Assoc. 1982, 180, 408–409.

    CAS  Google Scholar 

  • Baxby, D., N. Blundell & D. Hart: The development and performance of a simple sensitive method for the detection of Cryptosporidium oocysts in feces. J. Hyg. (Camb.) 1984, 92, 317–323.

    Article  Google Scholar 

  • Bennet, M., D. Baxbg, N. Blundell, C. J. Gaskell, C. A. Hart & D. F. Kelly: Cryptosporidiosis in a domestic cat. Vet. Rec. 1985, 116, 73–74.

    Article  Google Scholar 

  • Bogaerts, J., P. Lepage, D. Rouvroy & J. Vandepitte: Cryptosporidium spp., a frequent cause of diarrhea in central Africa. J. clin. Microbiol. 1984, 20, 874–876.

    Article  CAS  Google Scholar 

  • Bronsdon, M. A.: Rapid dimethyl sulfoxide - modified acid fast satin of Cryptosporidium oocysts in stool specimen. J. clin. Microbiol. 1984, 19, 952–953.

    Article  CAS  Google Scholar 

  • Campbell, P. N. & W. L. Current: Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections. J. clin. Microbiol. 1983, 18, 165–169.

    Article  CAS  Google Scholar 

  • Center for Disease Control: Cryptosporidiosis: Assessment of chemotherapy of males with acquired immune deficiency syndropie (AIDS). MMWR 1982, 31, 589–592.

    Google Scholar 

  • Haikala, O. J., J. O. Kokkonen, M. K. Leinonen, T. Nurmi, R. Mantyjdrvi & H. K. Sarkkinen: Rapid detection of rotavirus in stool by Latex agglutination: Comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test. J. med. Virol. 1983, 11, 91–97.

    Article  CAS  Google Scholar 

  • Henriksen, Sv. Aa. & J. F. Pohlenz: Staining of cryptosporidia by a modified Ziehl-Neelsen technique. Acta vet. scand. 1981, 22, 594–596.

    CAS  PubMed  Google Scholar 

  • Hughes, J. H., A. V. Tuomari, D. R. Mann & V. V. Hamparian: Latex immunoassay for rapid detection of rotavirus. J. clin. Microbiol. 1984, 20, 441–447.

    Article  CAS  Google Scholar 

  • Hunt, D. A., R. Shannon, S. R. Palmer & A. E. Jephcott: Cryptosporidiosis in an urban community. Brit. med. J. 1984, 289, 814–816.

    Article  CAS  Google Scholar 

  • Jokipii, L., S. Pohjola & A. M. M. Jokipii: Cryptosporidium: a frequent finding in patients with pastrointestinal symptoms. Lancet 1983, 11, 358–360.

    Article  Google Scholar 

  • Jokipii, L., S. Pohjola & A. M. M. Jokipii: Cryptosporidiosis associated with traveling and giardiasis. Gastroenterology 1985, 89, 838–842.

    Article  CAS  Google Scholar 

  • Krogh, H. V. & Sv. Aa. Henriksen: Bovine Cryptosporidiosis in Denmark. 2. Cryptosporidia associated with neonatal calf diarrhea. Nord. Vet-Med. 1985, 37, 42–47.

    CAS  PubMed  Google Scholar 

  • Lasser, K. H., K. J. Lewin & F. W. Ryning: Cryptosporidia! enteritis in a patient with congenital hypogammaglobulinemia. Hum. Pathol. 1979, 10, 234–240.

    Article  CAS  Google Scholar 

  • Mata, L., H. Bolanos, D. Pizarro & M. Vives: Cryptosporidiosis in children from some highland Costa Rican rural and urban areas. Amer. J. trop. Med. Hyg. 1984, 33, 24–29.

    Article  CAS  Google Scholar 

  • Meisel, J. L., D. R. Perera, C. Meligro & C. E. Rubin: Overwhelming watery diarrhea associated with cryptosporidium in an immunosuppressed patient. Gastroenterology 1976, 70, 1156–1160.

    Article  CAS  Google Scholar 

  • Miller, R. A., R. E. Holmberg & C. R. Clausen: Life-threatening diarrhea caused by Cryptosporidium in a child undergoing therapy for acute lymphocytic leukemia. J. Pediat. 1983, 103, 256–259.

    Article  CAS  Google Scholar 

  • Nime, F. A., J. D. Burek, D. L. Page, M. A. Holscher & J. H. H. Yardley: Acute enterocolitis in a human being infected with protozoan Cryptosporidium. Gastroenterology 1976, 70, 592–598.

    Article  CAS  Google Scholar 

  • Pohjola, S.: Negative staining method with nigrosin in detection of Cryptosporidia! oocysts: a comparative study. Res. Vet. Sci. 1983, 36, 217–219.

    Article  Google Scholar 

  • Pohjola, S., L. Jokipii & A. M. M. Jokipii: Dimethylsulphoxide-Ziehl- Neelsen staining technique for detection of cryptosporidial oocysts. Vet. Rec. 1984, 115, 442–443.

    Google Scholar 

  • Pohjola, S., H. Oksanen, E. Neuvonen, P. Veijalainen & K. Henriksson: Certain enteropathogens in calves of Finnish dairy herds with recurrent outbreaks of diarrhea. Prev. Vet. Med. 1986, 3, 547–558.

    Article  Google Scholar 

  • Pohjola, S., H. Oksanen, L. Jokipii & A. M. H. Jokipii: Outbreak of cryptosporidiosis among veterinary students. Scand. J. inf. Dis., in print.

  • Pohlenz, J., H. W. Moon, N. F. Cheville & W. J. Bembrick: Cryptosporidiosis as a probable factor in neonatal diarrhea in calves. J. Amer. vet. med. Assoc. 1978, 172, 452–457.

    CAS  Google Scholar 

  • Rahaman, A. S. M. H., S. C. Sanyal, K. A. Al-Mahmud, A. Sobhan, K. S. Hossain & B. C. Anderson: Cryptosporidiosis in calves and their handlers in Bangladesh. Lancet 1984, ii, 221.

    Article  Google Scholar 

  • Reese, N. C., W. L. Current, J. V. Ernst & W. S. Bailey: Cryptosporidium in man and calf: a case report and results of experimental infections in mice and rats. Amer. J. trop. Med. Hyg. 1982, 31, 226–229.

    Article  CAS  Google Scholar 

  • Schultz, M. G.: Emerging zoonoses. N. Engl. J. Med. 1983, 308, 1285–1286.

    CAS  Google Scholar 

  • Sloper, K. S., R. R. Dourmashkin, R. B. Bird, G. Slavin & A. D. B. Webster: Chronic malabsorption due to cryptosporidiosis in a child with immunoglobulin deficiency. Gut 1982, 23, 80–82.

    Article  CAS  Google Scholar 

  • Stein, E., J. Boch, J. Heine & G. Henkel: Der Verlauf natiirlicher Cryp- tosporidium-Infektionen in vier Rindersucht betrieben. (Course of natural Cryptosporidium infections on 4 cattle-breeding farms). Berl. Munch, tierarztl. Wschr. 1983, 96, 222–225.

    CAS  Google Scholar 

  • Stemmermann, G. N., T. Hyashi, G. A. Glober, N. Oishi & R. I. Frankel: Cryptosporidiosis, report of a fatal case complicated by disseminated toxoplasmosis. Amer. J. Med. 1980, 69, 637–642.

    Article  CAS  Google Scholar 

  • Tzipori, S. & I. Campbell: Prevalence of Cryptosporidium antibodies in 10 animal species. J. clin. Microbiol. 1981, 14, 455–456.

    Article  CAS  Google Scholar 

  • Tzipori, S.: Cryptosporidiosis in animals and humans. Microbiol. 1983, 47, 84–96.

    CAS  Google Scholar 

  • Tzipori, S., M. Smith, C. Birch, G. Barnes & R. Bishop: Cryptosporidiosis in hospital patients with gastroenteritis. Amer. J. trop. Med. Hyg. 1983, 32, 931–934.

    Article  CAS  Google Scholar 

  • Weinstein, L. S. M. Edelstein, J. L. Madara, K. R. Falchuk, B. M. Me Manus & J. S. Trier: Intestinal cryptosporidiosis complicated by disseminated cytomegalovirus infection. Gastroenterology 1981, 81, 584–591.

    Article  CAS  Google Scholar 

  • Weisburger, W. R., D. F. Hutcheon, J. H. Yardley, J. C. Roche, W. D. Hillis & P. Charade: Cryptosporidiosis in an immunosuppressed renal-transplant recipient with IgA deficiency. Amer. clin. Pathol. 1979, 72, 473–478.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food and Environmental Hygiene, College of Veterinary Medicine, P. O. Box 6, SF-00551, Helsinki, Finland

    Suvi Pohjola, Erkki Neuvonen, Aimo Niskanen & Annikka Rantama

Authors
  1. Suvi Pohjola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Erkki Neuvonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aimo Niskanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Annikka Rantama
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pohjola, S., Neuvonen, E., Niskanen, A. et al. Rapid immunoassay for detection of Crypto-sporidium oocysts. Acta Vet Scand 27, 71–79 (1986). https://doi.org/10.1186/BF03548560

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548560

Acta Veterinaria Scandinavica

ISSN: 1751-0147