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Colicin resistance in relation to pathogenicity factors in strains of Escherichia coli isolated from the intestinal tract of piglets

Colicin resistens relatert til enteropatogenitets faktorer hos stammer av Escherichia coli isolert fra tarmkanalen hos spedgris

Acta Veterinaria Scandinavica volume 27, pages 115–123 (1986)Cite this article

Abstract

Three hundred and fifteen E. coli strains isolated from the intestine of piglets were examined for K-antigens 88 and 99, enterotoxin production and colicin resistance. Of these strains 308 belonged to one of 3 following different groups: Group 1: 0149, K88, producing heat-labile (LT) and heat-stable (ST) enterotoxins, group 2: 064, K99, producing ST, and group 3: variable O-antigens, no K-antigens or enterotoxin production.

Almost 100 % of the E. coli strains were found to be resistant to colicins E1, E3, Ia, H and D+X. Resistance to colicins E2, B+M, V and K+X were found in 91.7 %, 43.8 %, 49.8 % and 62.2 % respectively.

E. coli strains in group 1 were always (resistant to colicin E2, while about 87 % of the other strains were resistant to this colicin. E. coli strains in group 2 were more often resistant to colicin B+M, V and K+X (65 %, 94 %, 83 %) than strains in group 1 (37 %, 24 %, 64 %) and strains in group 3 (37 %, 52 %, 46 %).

E. coli strains in group 2 showed a high degree of multiresistance, 45.1 % of the strains being resistant to all of the 9 colicins. About 10 % of the other strains were resistant to all of the 9 colicins.

E. coli strains harbouring the enteropathogenicity factors K99 antigen and ST production, showed a higher degree of colicin resistance than both the E. coli strains with K88 antigen and ST and LT production, and the E. coli strains lacking enteropathogenicity factors.

Sammendrag

Forekomst av K-antigen 88 og 99, enterotoxin produksjon og colicin resistens ble undersøkt hos 315 E. coli-stammer isolert fra tynntarmen hos spedgris. Av disse stammene tilhørte 308 stammer en av følgende tre grupper: Gruppe 1: O149, K88, produksjon av både varmelabilt (LT) og varmestabilt (ST) enterotoxin, gruppe 2: 064, K99, ST produksjon eller gruppe 3: varierende O antigen uten påvisbare K-antigen eller enterotoxin produksjon.

Tilnærmet 100 % av alle stammene var resistente mot colicinene E1, E3, Ia, H og D+X. Resistens mot colicinene E2, B+M, V og K+X ble påvist hos henholdsvis 91,7 %, 43,8 %, 49,8 % og 62,2 % av stammene.

Alle E. coli-stammer i gruppe 1 var resistente mot colicin E2, mens omtrent 87 % av de resterende stammer var resistente mot colicin E2. Stammer i gruppe 2 var oftere resistente mot colicin B + M, V og K + X (65 %, 94 %, 83 %) enn stammer i gruppe 1 (37 24 %, 64 %) og stammer i gruppe 3 (37 %, 52 %, 46 %).

E. coli-stammer i gruppe 2 var ofte resistente mot flere coliciner, 45,1 % av disse stammene var resistente mot alle de ni undersøkte colicinene. Tilnærmet 10 % av de resterende stammene var resistente mot alle ni coliciner.

E. coli-stammer med enteropatogenitets faktorene K99 antigen og ST produksjon var i større grad resistente mot coliciner enn stammer som hadde K88 antigen, ST og LT produksjon og stammer uten enteropatogenitets faktorer.

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Acknowledgments

The excellent technical assistance of Gry Jaeger and Mona Gjest-vang is highly appreciated.

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  1. National Veterinary Institute, P.O. Box 8156, N-0033, Oslo 1, Norway

    Berit K. Djϕnne

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  1. Berit K. Djϕnne
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Djϕnne, B.K. Colicin resistance in relation to pathogenicity factors in strains of Escherichia coli isolated from the intestinal tract of piglets. Acta Vet Scand 27, 115–123 (1986). https://doi.org/10.1186/BF03548564

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Acta Veterinaria Scandinavica

ISSN: 1751-0147