- Published:
The Production and Some Properties of the Brown Pigment of Aeromonas Liquefaciens
Produksjon og noen egenskaper hos det brune pigment fra Aeromonas liquefaciens
Acta Veterinaria Scandinavica volume 13, pages 251–259 (1972)
Abstract
The optimum conditions for pigment production by Ae. liquefaciens was found to be 30 °C and aerobic incubation, and the properties of the pigment are shown to be similar to those of the synthetic dopa-melanin referred to. The enzyme phenol oxidase was demonstrated in the culture filtrates, but not in extracts of disintegrated cells. As precursors for the pigment production, which was shown to be strictly pH dependent, DL-tyrosine, DL-dopa and catechol were used with success, while numerous other amino acids failed. The significance of the pigment as a criterium for the identification of strains which are pathogenic to certain animals or fishes, is not known.
Sammendrag
De optimale atmosfære- og temperaturforhold for pigmentproduksjon hos Ae. liquefaciens ble funnet å være aerob inkubasjon ved 30 °G, og pigmentets egenskaper tilsvarer egenskapene til det syntetiske dopamelanin som det refereres til. Enzymet phenoloxidase ble påvist i kulturfiltrat av Ae. liquefaciens, men ikke i ekstrakt av knuste bakterieceller. Som byggesteiner for pigment-produksjonen ble DL-tyrosin, DL-dopa og catechol brukt med suksess, mens flere andre aminosyrer ikke induserte pigmentproduksjonen. Betydningen av pigmentet som kriterium for identifikasjon av stammer som er patogene for enkelte dyr eller fisk er ikke kjent.
References
Blackberg, S. N. & J. O. Wanger: Melanuria. J. Amer. med. Ass. 1933, 100, 334–336.
Carberry, J. T.: Ulcerative dermal necrosis of salmonicids in Ireland. Symp. zool. Soc., London 1968, No. 24, 39–49.
Cooper, J. A. & F. Christine Brown: A rapid semi-quantitative method for the assay of tyrosinase activity. Clin. chim. Acta 1956, 1, 301–304.
Dahle, H. K.: Enzymoserological separation of bacterial proteinases. Acta path, microbiol. scand. 1969, 76, 143–148.
Dahle, H. K.: Regulation of the proteinase production in two strains of Aeromonas. Acta path, microbiol. scand. 1971, 79, 739–746.
Fox, D. L.: Animal Biochromes and Structural Colours; Physical, Chemical, Distributional and Physiological Features of Coloured Bodies in the Animal World. Cambridge Univ. Press, London and New York 1953.
Griffin, Philip J., S. F. Snieszko & S. B. Friddle: Pigment formation by Bacterium salmonicida. J. Bact. 1953, 65, 652–659.
Holt, G. & T. Hastein: Furunculosis in fish in Norway. Nord. Vet.- Med. 1970, 22, 505–509.
Lerner, A. B. & T. B. Fitzpatrick: Biochemistry of melanin formation. Physiol. Rev. 1950, 30, 91–126.
Mason, H. S.: Classification of melanins. N.Y. Acad. Sei. Spec. Publ. 1948, 4, 399–405.
Mason, H. S.: Comparative biochemistry of the phenolase complex. Advanc. Enzymol. 1955, 16, 105–184.
Masonti S.: Structure of melanins. In Gordon, M., ed.: Pigment Cell Biology. Acad. Press, New York 1959, 563–582.
Mencher, J. R. & A. H. Heim: Melanin biosynthesis by Streptomyces lavendulae. J. gen. Microbiol. 1962, 28, 665–670.
Meyer, F. P.: Field treatments of Aeromonas liquefaciens in golden strimers. Progve Fish Cult. 1964, 26, 33–35.
Rock, L. F. & H. M. Nelson: Charmel catfish and gizzard shad mortality by Aeromonas liquefaciens. Progve Fish Cult. 1965, 27, 138–141.
Ross, A. J.: Isolation of a pigment producing strain of Aeromonas liquefaciens from silver salmon (Oncorhynchus kisutch). J. Bact. 1962, 84, 590–591.
Yasunobu, K. T.: Mode of action of tyrosinase. In Gordon, M., ed.: Pigment Cell Biology. Acad. Press, New York 1959, 583–608.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aurstad, K., Dahle, H.K. The Production and Some Properties of the Brown Pigment of Aeromonas Liquefaciens. Acta Vet Scand 13, 251–259 (1972). https://doi.org/10.1186/BF03548579
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03548579