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Quantitative Determination of Bovine Plasma Fibrinogen by Rocket Immunoelectrophoresis and by Refractometry after Heat-Precipitation

Kvantitativ bestämning av bovint plasma fibrinogen med raketimmunoelektrofores och med refraktometri efter värmeprecipitation

Abstract

A standard syneresis method for measuring plasma fibrinogen concentrations in cattle was compared with a rocket Immunoelectrophoresis method and with a simple refractometer method; the correlations between the results obtained by the standard method and by the other 2 methods were high (r = 0.94 and 0.89 respectively, p < 0.001). The immunoelectrophoretic method is efficient and simple to perform. In contrast with the standard syneresis method, the other methods allow for the fibrinogen degradation products produced by fibrinolysis in vitro; they can therefore tolerate longer sample storage before analysis. The refractometer method is exceptionally simple and rapid and can be recommended for use under field conditions.

Sammendrag

En standard syneresmetod för bestämning av plasmafibrinogen hos nötkreatur jämfördes med raketimmunoelektroforesbestämning och med en enkel refraktometermetod; korrelationen mellan standardmetoden och de andra metoderna var god (r = 0,94 och 0,89 respektive, p < 0.001). Den immunoelektroforetiska metoden visades vara en effektiv och enkel laboratoriemetod for fibrinogenbestämning. Refraktometermetoden är utomordentligt enkel och snabb och får anses specielt lämplig för bestämningar under fältmässiga förhållanden.

I motsats till standard syneresmetoden tar de andra metoderna hänsyn till fibrinogennedbrytnings-produkter uppkomna genom fibrinolys in vitro.

Användandet av dessa metoder kan således tillåta längre lagringstider av proverna med viss in vitro nedbrytning av fibrinogenet och ändå ge en korrekt spegling av ursprungskoncentrationen av intakt fibrinogen i provet.

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Liberg, P. Quantitative Determination of Bovine Plasma Fibrinogen by Rocket Immunoelectrophoresis and by Refractometry after Heat-Precipitation. Acta Vet Scand 28, 271–277 (1987). https://doi.org/10.1186/BF03548593

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Keywords

  • cow
  • refractometer method
  • inflammation