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Neurotoxic Effects of Prenatal Trichlorfon Administration in Pigs
Nevrotoksiske effekter forƄrsaket av prenatal administrasjon av triklorfon til gris
Acta Veterinaria Scandinavica volumeĀ 28,Ā pages 321ā332 (1987)
Abstract
Pregnant sows were administered trichlorfon (NeguvonĀ®) during gestation. Examination of the offspring revealed clinical symptoms which included ataxia and tremor. The weights of the cerebrum and the celebellum were significantly reduced (67% and 41% of the control values). There was a clear correlation between the decrease in total brain weight and decrease in cerebellar weight (r = 0.94). Histological examination showed a well preserved lamination of the cerebral and cerebellar cortex. Periodical loss of Purkinje cells was the most notable finding in the cerebellum. The molecular layer in such regions was markedly thinned. Neurochemical investigations of the cerebellum showed a significant decrease in specific activity of the transmitter synthesizing enzymes choline acetyltransferase, glutamate decarboxylase and aromatic amino acid decarboxylase (61.6%, 30.3% and 47.1% of the control values, respectively). No effect in high affinity D-aspartate uptake was observed.
Sammendrag
Drektive purker ble gitt triklorfon (NeguvonĀ®) oralt pĆ„ dag 71, 77, 87 og 98 etter bedekning. Grisungene viste symptomer som ataksi og tremor. Vektene av cerebrum og cerebellum var signifikant redusert og utgjorde henholdsvis 67% og 41% av kontrolldyrene. Det var klar korrelasjon mellom reduksjonen i total hjernevekt og reduksjonen i vekten av cerebellum (r = 0.94). Histologiske undersĆøkelser viste en tydelig laminering av cortex bĆ„de i cerebrum og cerebellum. Stedvis tap av Purkinje celler ble observert i cerebellum.
Nevrokjemiske undersĆøkelser av cerebellum viste signifikant reduksjon i aktiviteten av de transmittor syntetiserende enzymene kolin acetyltransferase, glutamat dekarboksylase og aromatisk aminosyre dekarboksylase (henholdsvis 30,4%, 69,7% og 52,9% av kontrollverdiene). Ingen effekt pĆ„ hĆøy affinitet D-aspartat opptak ble observert.
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Acknowledgment
The authors wish to express their sincere thanks to professor Erling Sognen and professor Inger Nafstad for useful help, valuable advice and comments. The technical assistance of Mrs. E. Iversen, Mrs. Sidsel Sohlberg and Mr. T. Myrvold is greatly appreciated. This work was supported by grants from the Norwegian Agricultural Research Council.
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Berge, G.N., Fonnum, F. & Brodai, P. Neurotoxic Effects of Prenatal Trichlorfon Administration in Pigs. Acta Vet Scand 28, 321ā332 (1987). https://doi.org/10.1186/BF03548600
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DOI: https://doi.org/10.1186/BF03548600