Skip to main content
Download PDF

Pulmonary Multinucleate Giant Cells in Dermatosis Vegetans in Swine: Light microscopic and immunohistochemical investigations

Pulmonære multinukleære kjempeceller ved dermatosis vegetans hos gris. Lysmikroskopiske og immunhistokjemiske studier

Acta Veterinaria Scandinavica volume 28, pages 429–433 (1987)Cite this article

Abstract

Five pigs with dermatosis vegetans (DV), aged 1–28 days, were examined with the purpose of describing the pulmonary changes and to characterize the pulmonary multinucleate giant cells (MGC) and their possible cytogenesis.

No pulmonary changes were present at birth. From 7 days of age, lung changes were characterized by proliferation of alveolar epithelial cells and formation of MGCs. Immunostaining for cytokeratin by a peroxydase–streptavidin method gave a positive reaction in MGCs, bronchial, bronchiolar and alveolar epithelium. MGCs seemed to be formed in the course of alveolar epithelial proliferations, and type-II pneumocytes were proposed as possible precursors.

Sammendrag

Fem griser i alderen 1 til 28 dager med lidelsen dermatosis vegetans (DV) ble undersøkt med henblikk på å beskrive lungeforandringer og spesielt for å gi en morfologisk karakterisering av pulmonære multinukleære kjempeceller (PMK) og deres mulige cytogenese.

Det forelå ingen lungeforandringer ved fødsel. Fra 7 dagers alder var lungeforandringene karakterisert ved proliferasjon av alveole-epitelceller og PMK-dannelser. Ved immunhistokjemiske studier basert på en peroksydase-streptavidin metode ble det påvist positiv reaksjon i PMK, bronkie-, bronkiole- og proliferert alveole epitel.

PMK syntes å dannes i forløpet av alveolære proliferasjoner og type-II pneumocytter ansees som en mulig cellulær opprinnelse.

References

  • Barry Β Ε, Wong Κ C, Brody A R, Crapo J D: Reaction of rat lungs to inhaled chrysotile asbestos following acute and subchronic exposures. Exp. Lung Res. 1983,5, 1–22.

    Article  CAS  Google Scholar 

  • Blobel G A, Moll R, Franke W W, Vogt-Maykopf I: Cytokeratins in normal lung and lung carcinomas. Virch. Arch. (Cell Path.) 1984,45, 407–429.

    Article  CAS  Google Scholar 

  • Bryson DG, McNulty Μ S, Ball H J, Neill S D, Connor Τ J, Cush P F: The experimental production of pneumonia in calves by intranasal inoculation of parainfluenza type 3 virus. Vet. Rec. 1979,105, 566–573.

    CAS  PubMed  Google Scholar 

  • Bryson D G, McNulty Μ S, Logan Ε F, Cush Ρ F: Respiratory syncytial virus pneumonia in young calves: Clinical and pathologic findings. Amer. J. vet. Res. 1983,44, 1648–1655.

    CAS  PubMed  Google Scholar 

  • Done J T, Loosmore R M, Saunders C N: Dermatosis vegetans in pigs. Vet. Rec. 1967,80, 292–298.

    Article  CAS  Google Scholar 

  • Evans Μ J, Dekker N P, Cabral-Anderson L J, Freeman G: Quantitation of damage to the alveolar epithelium by means of type 2 cell proliferation. Amer. Rev. Resp. Dis. 1978,118, 787–790.

    Article  CAS  Google Scholar 

  • Flatla J L, Hansen M A, Slagsvold P: Dermatosis vegetans in pigs. Symptomatology and genetics. Zbl. Vet. Med. 1961,8, 25–42.

    Google Scholar 

  • Jericho Κ W F: Dermatosis vegetans - giant cell pneumonitis in pigs: Further observations and interpretations. Res. Vet. Sci. 1974,16, 176–181.

    Article  CAS  Google Scholar 

  • Mariano M, Spector W G: The formation and properties of macrophage polykaryons (Inflammatory giant cells). J. Path. 1974,113, 1–19.

    Article  CAS  Google Scholar 

  • Moll R, Franke W W, Schiller L, Geiger B, Krepler R: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982,31, 11–24.

    Article  CAS  Google Scholar 

  • Papadimitriou J M, Walters M N-I: Macrophage po–lykarya. CRC Crit. Rev. Tox. 1979, 211–255.

    Google Scholar 

  • Percy DH, Hulland Τ J: Evolution of multinucleate giant cells in dermatosis vegetans in swine. Path, vet. 1968,5, 419–428.

    CAS  Google Scholar 

  • Schlegel R, Banks-Schlegel S, Pinkus G S: Immuno–histochemical localization of keratin in normal human tissues. Lab. Inv. 1980,42, 91–96.

    Google Scholar 

  • Shami S G, Martinez L A, Evans Μ J: The role of migrating inflammatory cells in proliferation of lung interstitium and epithelium. Chest 1986, 89, suppl. 171–173.

    Google Scholar 

  • Vogel A M, Gown A M: Monoclonal antibodies to intermediate filament proteins. In Shay, J. W. ed. Cell and muscle motility. Vol. 5, Plenum Press, New York, 1984, pp. 379–402.

    CAS  PubMed  Google Scholar 

  • Winkler G C, Cheville N F: The neonatal lung: Ultra–structural morphology and postnatal development of the terminal airways and alveolar region. Anat. Rec. 1984,210, 303–313.

    Article  CAS  Google Scholar 

  • Woodcock-Mitchell J L, Burkhardt A L, Mitchell SR, Rannels D, Rannels E, Chiu J-F, Low R: Keratin species in type II pneumocytes in culture and during lung injury. Amer. Rev. Resp. Dis. 1986,34, 566–571.

    Google Scholar 

Download references

Acknowledgment

This study was financially supported by the Norwegian Agricultural Research Council.

Author information

Authors and Affiliations

  1. National Veterinary Institute, P. O. Box 8156 Dep., N-0033, Oslo 1, Norway

    Øystein Evensen & Bjørn Bratberg

Authors
  1. Øystein Evensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bjørn Bratberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Evensen, Ø., Bratberg, B. Pulmonary Multinucleate Giant Cells in Dermatosis Vegetans in Swine: Light microscopic and immunohistochemical investigations. Acta Vet Scand 28, 429–433 (1987). https://doi.org/10.1186/BF03548611

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548611

Keywords

  • lungs
  • syncytia formations
  • cytokeratins
  • type-II pneumocytes

Acta Veterinaria Scandinavica

ISSN: 1751-0147