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Species Differentiation of DNA using Dot-Blots with Biotinylated or 32P-Labeled Genomic DNA Probe

Abstract

Recombinam DNA technologies offer new methods of detecting animal diversity at various levels. DNA »fingerprinting« using cloned repetitive sequences can be used to distinguish between individuals within breeds and may thus be used for paternity testing (Jeffreys et al. 1985). The forensic value of evidence of this kind has given guarded approval by the UK Immigration Advisory Service in its annual report (Nature 1987). At the between-species level labeled total genomic DNA was used by Durnam et al. (1985) as probe in an in situ hybridization for speciesverification of chromosomal materials in human-rodent hybrid cell lines, and we recently described the use of 3H-labeled genomic pig DNA probe for detection af swine chromosomes in swine-hamster hybrid cell lines (Thomsen & Christensen 1986).

References

  • Chan V T-W, Fleming Κ A, McGee J Ο’D: Detection of subpicogram quantities of specific DNA sequences on blot hybridization with biotinylated probes. Nucleic Acids Res. 1985,13, 8083–8091.

    Article  CAS  Google Scholar 

  • Durnam D M, Gelinas R E, Myerson D: Detection of Species Specific Chromosomes in Somatic Cell Hybrids. Somatic Cell Molec. Genet. 1985,11, 571–577.

    Article  CAS  Google Scholar 

  • Jeffreys A J, Wilson V, Thein S L: Individual-specific »fingerprints« of human DNA. Nature 1985,316, 76–79.

    Article  CAS  Google Scholar 

  • Kafatos F C, Jones C Wt Efstratiadis A: Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Research 1979,7, 1541–1552.

    Article  CAS  Google Scholar 

  • Maniatis T, Fritch E F, Sambrook J: Molecular cloning. Cold Spring Harbor Laboratory, New York 1982.

    Google Scholar 

  • Thomsen Ρ D, Christensen K: Detection of swine chromosomes in interspecies somatic cell hybrids by means of DNA–hybridization. Proc. 7th European Colloquium on Cytogenetics of Domestic Animals 1986, in press.

    Google Scholar 

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Acknowledgment

The authors wish to thank Mrs. I. Christensen and Miss S. J. Nielsen for excellent technical assistance and Dr. Zeegers, Duphar, for good cooperation and the extraction of cell line DNA. The work was supported by the Carlsberg Foundation, The Nørtoft Thomsen legat and The Danich Agricultural and Veterinary Research Council Grant No. 5.17.4.3.06.

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Dybdahl Thomsen, P., Christensen, K. Species Differentiation of DNA using Dot-Blots with Biotinylated or 32P-Labeled Genomic DNA Probe. Acta Vet Scand 28, 451–453 (1987). https://doi.org/10.1186/BF03548615

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  • DOI: https://doi.org/10.1186/BF03548615

Keywords

  • non–autoradiographic
  • dot–blot
  • genomic probe
  • species verification
  • cell lines