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Species Differentiation of DNA using Dot-Blots with Biotinylated or 32P-Labeled Genomic DNA Probe


Recombinam DNA technologies offer new methods of detecting animal diversity at various levels. DNA »fingerprinting« using cloned repetitive sequences can be used to distinguish between individuals within breeds and may thus be used for paternity testing (Jeffreys et al. 1985). The forensic value of evidence of this kind has given guarded approval by the UK Immigration Advisory Service in its annual report (Nature 1987). At the between-species level labeled total genomic DNA was used by Durnam et al. (1985) as probe in an in situ hybridization for speciesverification of chromosomal materials in human-rodent hybrid cell lines, and we recently described the use of 3H-labeled genomic pig DNA probe for detection af swine chromosomes in swine-hamster hybrid cell lines (Thomsen & Christensen 1986).


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The authors wish to thank Mrs. I. Christensen and Miss S. J. Nielsen for excellent technical assistance and Dr. Zeegers, Duphar, for good cooperation and the extraction of cell line DNA. The work was supported by the Carlsberg Foundation, The Nørtoft Thomsen legat and The Danich Agricultural and Veterinary Research Council Grant No.

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Dybdahl Thomsen, P., Christensen, K. Species Differentiation of DNA using Dot-Blots with Biotinylated or 32P-Labeled Genomic DNA Probe. Acta Vet Scand 28, 451–453 (1987).

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  • non–autoradiographic
  • dot–blot
  • genomic probe
  • species verification
  • cell lines