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Comparative Characterization and Biotyping of Staphylococcus aureus Isolates from Human and Bovine Sources

Sammenlignende beskrivelse og typebestemmelse af humane og bovine isolater af Staphylococcus aureus

Acta Veterinaria Scandinavica volume 29, pages 303–310 (1988)Cite this article

Abstract

One Hundred and ten alpha and/or delta-haemolytic isolates (collection 1), 50 beta haemolytic isolates (collection 2) from bovine mastitis, and 100 previously phage-typed alpha- and delta-haemolytic isolates (human collection) og Staphylococcus aureus (S. aureus) were tested and biotyped according to the scheme of Hajek & Marsalek (1971). Among collection 1 isolates, 85 (77.3 %) belonged to the human biotype A (human source). Twenty two (20 %) designated as non-allotted strains, possessed characteristics of both animal and human sources. The remaining 3 isolates (2.7 %) in this collection belonged to biotype C (animal source).

All collection 2 isolates which were used as control strains for animal sources, belonged to biotype C.

The human collection that contained 100 phage-typed haemolytic isolates (representing all human phage groups) were used as a control for the human source. Irrespective of their phage group, these strains predominantly produced alpha and/or delta haemolysins and belonged to the human biotype A. This study also recommended the use of a combined plasma crystal violet agar medium for the presumptive identification of S. aureus biotypes.

Sammendrag

110 alfa- og/eller deltahæmolytiske stammer (indsamling 1), 50 betahæmolytiske stammer (indsamling 2) fra mastitis hos kvæg og 100 tidligere fagtypede alfa- og deltahæmolytiske stammer (human indsamling) af Staphylococcus aureus blev undersøgt og biotypet efter Hajek og Marsaleks metode (1971).

85 (77,3 %) af stammerne i indsamling 1 tilhørte den humane biotype A (human oprindelse); 22 (20 %) såkaldte vilde stammer viste egenskaber hidrørende fra både animalske og humane kilder. de øvrige 3 isolater (2,7 %) i denne indsamlings-gruppe tilhørte biotype C (animalsk oprindelse). Alle 50 isolater i indsamling 2, der blev brugt som kontrolstammer for animalske kilder, tilhørte bio-type C.

Den tredje gruppe, der indeholdt 100 fagtypede hæmolytiske stammer (repræsenterende alle humane faggrupper), blev anvendt som kontrol for human oprindelse. Uanset faggruppe producerede disse stammer hovedsageligt alfa- og/eller delta-hæmolysiner og tilhørte den humane biotype A. Denne undersøgelse viser tillige, at anvendelse af kombineret plasma-krystalviolet-agarsubstrat kan anbefales til foreløbig identification af S. aureus biotyper.

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Acknowledgments

This work was supported by FAO/DANIDA under the administration of the Development Cooperation Bureau, Copenhagen, Denmark. We are very grateful to Dr. K. Rosendal of the Department of Hospital Infections, SSI, Denmark, for phage-typing the bovine mastitis isolates and providing the phage-typed human strains.

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  1. Development Cooperation Bureau, Royal Veterinary and Agricultural University, Bulowsvej 13, DK-1870, Frederiksberg C, Denmark

    Kaj Bruhn, Ibrahim O. Farah, Erik Pedersen & Christian Halgaard

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  1. Kaj Bruhn
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Bruhn, K., Farah, I.O., Pedersen, E. et al. Comparative Characterization and Biotyping of Staphylococcus aureus Isolates from Human and Bovine Sources. Acta Vet Scand 29, 303–310 (1988). https://doi.org/10.1186/BF03548622

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Acta Veterinaria Scandinavica

ISSN: 1751-0147