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Pre-Enrichment and Enrichment Methods for Isolating Small Number of Campylobacteria from Contaminating Flora

Preinkuberings- och anrikningsmetod till ett litet antal campylobacterier med kontaminering

Acta Veterinaria Scandinavica volume 29, pages 443–449 (1988)Cite this article

Abstract

A method was developed to detect fewer than 100 CFU of campylobacteria from SIFF transport medium to which thawing drip from deep frozen broiler carcasses was added as a source of contamination and which was then stored at room temperature for 20 h. The method was made possible by using pre–enrichment in 1 % buffered peptone water under a microaerophilic atmosphere for 5 h at 43°C before selective enrichment either in brucella enrichment broth and on brucella blood selective agar supplemented with Skirrow antibiotics or in CCD enrichment broth and on blood free CCD selective agar. The other pre–enrichment broth studied was alkaline peptone water with reducing agents (RAPW) and the other enrichment broths and selective agars were Preston broth and agar, THAL broth and alkaline tryptose broth (ATB) and brucella agar with ATB antibiotics. Contaminating flora can be a problem when using enrichment broths and selective agars with limited antibiotic supplementation.

Sammanfattning

Färre än 100 CFU av campylobakterier kunde isoleras ur SIFF transport medium som hade kontaminerats med smältvatten från djupfrysta broiler och som förvarats i rumstemperatur för 20 h. Metoden bestod av 5 timmar preinkubering i 1 % peptonvatten i mikroaerofil atmosfär i 43°C före det selektive anrikandet antingen i brucella buljong och på brucella blod agar med Skirrow antibiotika eller i kol-cefazolin-desoxycholate (CCD) anrikningsbuljong och på CCD agar. Den andra preinkuberingsbuljongen studerats var alkalisk pepton vatten med reducerande medel (RAPW) och andra buljonger och plattor var Preston-buljong och -medium, THAL-buljong och alkalisk trypton buljong (ATB) och brucella agar med ATB-antibiotika. Kontaminering av plattorna kan vara ett problem när man använder anriknings-buljonger och selektiva medier med begränsad dosering av antibiotika.

References

  • Blankenship LC, Craven LC: Campylobacter jejuni survival in chicken meat as a function of temperature. Appl. environ. Microbiol. 1982, 44, 88–92.

    Article  CAS  Google Scholar 

  • Blaser MJ, Berkowitz ID, LaForce FM, Cravens J, Reller LB, Wang W–LL: Campylobacter enteritis: clinical and epidemiolocical features. Ann. intern. Med. 1979, 91, 179–185.

    Article  CAS  Google Scholar 

  • Bolton FJ, Robertson L: A selective medium for isolating Campylobacter jejuni/coli. J. clin. Pathol. 1982, 35, 462–467.

    Article  CAS  Google Scholar 

  • Bolton FJ, Hutchinson DN, Coates D: Blood–free selective medium for isolating of Campylobacter jejuni from feces. J. clin. Microbiol. 1984, 19, 169–171.

    Article  CAS  Google Scholar 

  • Chan FTH, Mackenzie AMR: Enrichment medium and control system for isolation of Campylobacter fetus subsp. jejuni from stools. J. clin. Microbiol. 1982, 15, 12–15.

    Article  CAS  Google Scholar 

  • Doyle MP: Campylobacter in foods. In: Butzler JP (ed.): Campylobacter Infections in Man and animals. CRC Press, Inc., Boca Raton 1984, pp. 163–180.

    Google Scholar 

  • Humphrey TJ, Becket P, Cruickshank JG: A technique for the isolation of sub-lethally injured Campylobacter jejuni from foodstuffs and its application in a study of the influences of drinking water source and milking hygiene on the levels of C. jejuni in raw milk. In: Pearson AD, Skirrow MB, Lior H, Rowe Β (eds.): Campylobacter III. Public Health Laboratory Service, London 1985, pp. 64–65.

  • Humphrey TJ, Cruickshank JG: Sub–lethal injury and Campylobacters. In: Pearson AD, Skirrow MB, Rowe B, Davies JR, Jones DM (eds.): Campylobacter II, Proceedings of the Second International Workshop on Campylobacter Infections. Brussels, 6.–9. September 1983. Public Health Laboratory Service, London 1983, pp. 51–52.

  • Humphrey TJ, Cruickshank JG: Antibiotic and deoxycholate resistance in Campylobacter jejuni following freezing and thawing. J. appl. Bact. 1985,59,65–71.

    Article  CAS  Google Scholar 

  • Hänninen M–L: Survival of Campylobacter jejuni/ coli in ground refrigerated and ground frozen beef liver and in frozen broiler carcasses. Acta vet. scand. 1981, 22, 566–577.

    PubMed  Google Scholar 

  • Hanninen M–L: Effect of recovery medium on the isolation of Campylobacter jejuni before and after heat treatment. Acta vet. scand. 1982, 23, 416–424.

    Article  CAS  Google Scholar 

  • International Standardization Organization: Microbiology–general guidance on methods for the detection of Salmonella. ISO 6579-1981 (E).

  • Lai-King NG, Taylor DE, Stiles ME: Are Campylobacters always resistant to antibiotics used in selective media? In: Pearson AD, Skirrow MB, Lior H, Rowe Β (eds.): Campylobacter III. Public Health Laboratory Service, London 1985, pp. 61–62.

  • Moran AP, Upton ME: Factors affecting production of coccoid forms by Campylobacter jejuni on solid media during incubation. J. appl. Bact. 1987, 62, 527–537.

    Article  CAS  Google Scholar 

  • Oosterom J, Notermans S, Karman B, Engels GB: Origin and prevalence of Campylobacter jejuni in poultry processing. J. Food Prot. 1983, 46, 339–344.

    Article  CAS  Google Scholar 

  • Palumbo SA: Heat injury and repair in Campylobacter jejuni. Appl. environ. Microbiol. 1984, 48, 477–80.

    Article  CAS  Google Scholar 

  • Park CE, Stankiewicz ZK, Lovett J, Hunt J, Francis DW: Effect of temperature, duration of incubation and pH of enrichment culture on the recovery of Campylobacter jejuni from eviscerated market chickens. Can. J. Microbiol. 1983, 29, 803–806.

    Article  CAS  Google Scholar 

  • Ray B, Johnson C: Sensitivity of cold–stressed Campylobacter jejuni to solid and liquid selective environments. Food Microbiol. 1984a, 1, 173–176.

    Article  Google Scholar 

  • Ray B, Johnson C: Survival and growth of freeze–stressed Campylobacter jejuni cells in selective media. J. Food Safety 1984b, 6, 183–195.

    Article  Google Scholar 

  • Robinson DA: Infective dose of Campylobacter jejuni in milk. Brit. Med. J. 1981, 282, 1584.

    Article  CAS  Google Scholar 

  • Rollins DM, Colwell RR: Viable but noncultur–able stage of Campylobacter jejuni and its role in the natural aquatic environment. Appl. environ. Microbiol. 1986, 52, 513–538.

    Article  Google Scholar 

  • Sandven P, Solberg O, Ødegaard K, Myhre G: Improved medium for the transportation of gonococcal specimens. Acta pathol. microbiol. immunol. scand. Sect. B. 1982, 90, 73–77.

    CAS  Google Scholar 

  • Sjogren E, Lindblom G–B, Kaijser B: Optimal transport and enrichment methods for the isolation of Campylobacter jejuni. In: Pearson AD, Skirrow MB, Lior H, Rowe Β (eds.): Campylobacter III. Public Health Laboratory Service, London 1985, pp. 62–63.

    Google Scholar 

  • Skirrow MB: Campylobacter enteritis: a ”new” disease. Brit. Med. J. 1977, 2, 9–11.

    Article  CAS  Google Scholar 

  • Svedhem Å, Kaijser B, Sjögren E: The occurrence of Campylobacter jejuni in fresh food and survival under different conditions. J. Hyg., Camb. 1981, 87, 421–425.

    Article  CAS  Google Scholar 

  • Wang W-LL, Reller LB, Smallwood B, Luechte–feld NW, Β laser Μ J: Evaluation of transport media for Campylobacter jejuni in human fecal specimens. J. clin. Microbiol. 1983, 18, 803–807.

    Article  CAS  Google Scholar 

  • Wesley RD, Swaminathan B, Stadelman WJ: Isolation and enumeration of Campylobacter jejuni from poultry products by selective enrichment method. Appl. environ. Microbiol. 1983, 46, 1097–1102.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Hygiene, National Veterinary Institute, P. O. Box 368, SF-00101, Helsinki, Finland

    Matti Aho, Maija Kauppi & Jorma Hirn

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  1. Matti Aho
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  2. Maija Kauppi
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Aho, M., Kauppi, M. & Hirn, J. Pre-Enrichment and Enrichment Methods for Isolating Small Number of Campylobacteria from Contaminating Flora. Acta Vet Scand 29, 443–449 (1988). https://doi.org/10.1186/BF03548641

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Acta Veterinaria Scandinavica

ISSN: 1751-0147