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The Significance of NT1, NT2, and NT3 Antigens in Epidemiological Investigations of Bovine Group-B Streptococcus Infections

NT1, NT2 og NT3 antigenernes betydning ved epidemiologiske undersøgelser af gruppe B-streptokokinfektioner

Acta Veterinaria Scandinavica volume 22, pages 307–314 (1981)Cite this article

Abstract

On typing of 90 strains of bovine Group-B streptococci (B-str.) from 21 herds, NT1, NT2 and NT3 antigens were found in 30 %, viz., NT1 in 14.4 %, NT2 in 3.3 %, and NT3 in 12.2 %. In 9 of the 21 herds examined (43 %) one or other of these antigens was found.

Among 8 strains from 4 herds of Herd type NT the NT1 antigen was demonstrated once.

In 5 herds of Herd type X, 20 strains were typed, and 9 isolates (45 %) from 3 herds (60 %) carried one or other of the three antigens.

Among herds in which the herd type was referable to polysaccharide antigens of the accepted set, NT3 antigen was present in 1 herd of Herd type Ia, namely along with IaX antigens in two strains, and with Ia antigen in one. In 3 herds of Herd type Ibc and in 2 herds of Herd type Ib no NT1, NT2, or NT3 antigen was demonstrated. On the other hand, such antigens were found in all of 4 herds of Herd type III, being present in 50 % of the strains (14/28) examined, and the frequency of NT1, NT2, and NT3 antigens was only slightly higher among NT and X strains (10/19 or 53 %) than among III and IIIX strains (4/9 or 44 %).

The NT2 reference strain carried the Ibc protein antigen. In addition to strong homologous reactions, NT1 antiserum reacted with B-str. group antigen, NT2 antiserum with Ibc antigen, and NT3 antiserum with the NT1 reference strain.

It is concluded that the NT1, NT2, and NT3 antigens are of doubtful value in epidemiological studies on bovine infections with group-B streptococci.

Sammendrag

Ved serologisk typebestemmelse af 90 bovine grouppe B-streptokokstammer (B-str.) hidrørende fra 21 besætninger fandtes prævalensen af NT1, NT2 og NT3 antigenerne til 30% (NT1: 14,4%, NT2: 3,3 %, NT3: 12,2 %). Det ene eller det andet af disse antigener påvistes i 9 af de 21 besætninger (43 %).

Blandt 4 besætninger af besætningstype NT påvistes NT1 antigenet i eet tilfælde.

Blandt 20 stammer hidrørende fra 5 besætninger af besætningstype X påvistes NTl, NT2 eller NT3 på 9 stammer (45 %) med oprindelse i 3 af de undersøgte besætninger.

Fra besætninger, hvor besætningstypen var baseret på forekomst af polysakkerid- eller Ibc proteinantigenet påvistes NT3 antigenet på 3 stammer i en besætning af besætningstype Ia (3 undersøgte besætninger). NT1, NT2 eller NT3 påvistes derimod ikke blandt isolater fra 3 besætninger af besætningstype Ibc og 2 af besætningstype Ib.

Disse antigener påvistes i alle 4 undersøgte besætninger af besætningstype III, og 50 % af isolaterne (14/28) var positive. Inden for denne besætningskategori var der kun lille forskel i frekvensen af NT1, NT2 og NT3 blandt NT og X stammer (10/19 eller 53 %) og III og IIIX stammer (4/9 eller 44 %).

Referencestamme NT2 var i besiddelse af Ibc proteinantigenet. Foruden stærke homologe reaktioner reagerede antiserum NT1 med B-str. gruppeantigen, NT2 med proteinantigenet Ibc og NT3 antiserum med NT1 antigen.

Det konkluderes, at NT1, NT2 og NT3 antigenerne indtil videre må anses for at være af tvivlsom værdi ved epidemiologiske undersøgelser af bovine B-str. infektioner.

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  1. State Veterinary Serum Laboratory, Odinsvej, DK-4100, Ringsted, Denmark

    Niels Einar Jensens & Burghild Berg

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  1. Niels Einar Jensens
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  2. Burghild Berg
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Jensens, N.E., Berg, B. The Significance of NT1, NT2, and NT3 Antigens in Epidemiological Investigations of Bovine Group-B Streptococcus Infections. Acta Vet Scand 22, 307–314 (1981). https://doi.org/10.1186/BF03548656

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Acta Veterinaria Scandinavica

ISSN: 1751-0147