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An Enzyme Histochemical Investigation of the Intestinal Mucosa in Diarrheic Calves

En enzym-histokjemisk undersøkelse av tarmmukosa hos kalver med diaré

Acta Veterinaria Scandinavica volume 22, pages 449–458 (1981)Cite this article

Abstract

Selected enzymes were examined in the small intestine of twelve 2–5 week-old calves, 8 with diarrhea and 4 convalescents. The diarrheic calves showed a reduction of enzyme reactions mainly in the duodenum and middle small intestine, and the crypt reactions appeared most severely affected. In the duodenum, villous alkaline phosphatase, adenosine triphosphate-(ATP)-splitting enzyme, and β-D-galactosidase were reduced in 3 calves; the reaction in the corresponding crypts was decreased in 6 calves for the ATP-splitting enzyme and in 4 calves for the β-D-galactosidase. Six calves showed decrease of villous brush border acid phosphatase, and 3 of villous non-specific esterase. In the middle jejunum, villous ATP-splitting enzyme was reduced in 3 calves, while 5 showed decrease of the corresponding crypt reaction. Convalescents had no enzyme reduction in the duodenum, whereas 1 showed marked reduction of the ATP-splitting enzyme and aminopeptidase in the middle and posterior jejunum. The decreased enzyme reactions in the present material may be caused by immaturity of epithelial cells associated with regenerative crypt hyperplasia and/or microbial destruction of enzymes.

Sammendrag

Et utvalg av enzymer i tynntarmsmukosa ble studert hos tolv 2–5 uker gamle kalver, 8 med diaré og 4 rekonvalesenter. Diarékalvene viste reduksjon av enzymene spesielt i duodenum og midtre jejunum; krypt-reaksjonene syntes å være sterkest affisert. I duodenum var alkalisk fosfatase, adenosintrifosfat (ATP)-spaltende enzym og β-D-galaktosidase langs villi redusert hos 3 kalver; reaksjonen i de tilsvarende kryptene var nedsatt hos henholdsvis 6 kalver når det gjaldt ATP-spaltende enzym og 4 når det gjaldt β-D-galaktosidase. Seks kalver viste reduksjon av sur fasfatase i „børstesømmen“ av villi og 3 hadde nedgang i reaksjonen av uspesifikk esterase i villi. I midtre jejunum var ATP-spaltende enzym langs villi redusert hos 3 kalver, mens 5 viste nedgang i den tilsvarende krypt-reaksjonen. Rekonvalesentene viste ingen nedgang av enzym-reaksjoner i duodenum, men i midtre og bakre jejunum var det en markert reduksjon av ATP-spaltende enzym og aminopeptidase hos én kalv. De nedsatte enzymreaksjoner i dette materialet kan skyldes en mangelfull differensiering av epitelcellene forbundet med regenerativ hyperplasi i kryptene og/eller mikrobiell destruksjon av enzymene.

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Authors and Affiliations

  1. Department of Pathology, Veterinary College of Norway, P.O. Box 8146, Dep., Oslo 1, Norway

    Thor Landsverk

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  1. Thor Landsverk
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This study was supported by grants from the Agricultural Research Council of Norway.

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Landsverk, T. An Enzyme Histochemical Investigation of the Intestinal Mucosa in Diarrheic Calves. Acta Vet Scand 22, 449–458 (1981). https://doi.org/10.1186/BF03548670

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Acta Veterinaria Scandinavica

ISSN: 1751-0147