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Effects of Different Anticoagulants on Determination of Erythrocyte Glutathione Peroxidase

Effekt av olika antikoagulantia på beståmning av glutathionperoxidas i erytrocyter

Acta Veterinaria Scandinavica volume 22, pages 472–479 (1981)Cite this article

Abstract

Glutathione peroxidase (GSH-Px) was determined in whole blood from cows, goats and horses using cumenehydroperoxide as substrate. Heparin was found to be the most suitable anticoagulant. The highest activities of GSH-Px were found with high concentrations of heparin in the blood samples (1000 and 1250 IU/ml of blood). Sodium fluoride and especially EDTA and sodium citrate gave lower activities of the enzyme.

Storage of the blood samples at room temperature (~20°C) or in a refrigerator (~5°C) for 3 days resulted in significantly lower activities of the enzyme, especially in horse blood.

Sammanfattning

Glutationperoxidas (GSH-Px) bestämdes i helblod från kor, getter och hästar. Cuminhydroperoxid användes som substrat vid bestämningarna som utfördes i en LKB 8600 Rection Rate Analyzed. Heparin var det mest lämpliga antikoagulantium som testades. De högsta aktiviteterna av enzymet uppmättes då höga halter av heparin fanns i blodprovet (1000 och 1250 ΙΕ/ml blod). Natriumfluorid men specieilt EDTA och natriumcitrat gav lägre enzymaktiviteter vid samtidig bestämning på samma blodprov.

Förvaring av blodp roverna i rumstemperatur (20°C) eller i kylskåp (5°C) under 3 dagar gav signifikant lägre enzymaktiviteter. Detta var specieilt tydligt i hästblod.

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Authors and Affiliations

  1. Department of Clinical Chemistry, College of Veterinary Medicine, Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden

    Kamal S. M. Hussein & Bernt-E. V. Jones

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  1. Kamal S. M. Hussein
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  2. Bernt-E. V. Jones
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Hussein, K.S.M., Jones, BE.V. Effects of Different Anticoagulants on Determination of Erythrocyte Glutathione Peroxidase. Acta Vet Scand 22, 472–479 (1981). https://doi.org/10.1186/BF03548672

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Acta Veterinaria Scandinavica

ISSN: 1751-0147